Study On The Chromatography Method For The Residue Determination Of Three Types Of Plant Growth Regulators In Apple

Plant growth regulators have wide range of applications in modern agriculture, forestry, fruit trees, vegetables and flower productions. They bring enormous benefits to people's production and life. As the increase awareness of environmental protection, people not only focus on crop yields and nutrition, but also concern about the environment and food safety issues. Therefore, the analyses of plant growth regulators have important significance.With high performance liquid chromatography (HPLC) and ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), the three types of plant growth regulators analytical methods have been researched and established in this paper, which contain the following three aspects:We established a practical method for the separation and determination of forchlorfenuron, chlorantraniliprole and diflubenzuron by HPLC-UV. By the optimization of chromatographic separation column, mobile phase and the wavelength, three substances achieved baseline separation within 8 minutes. The detection limits were 0.02μg/mL,0.05μg/mL and 0.13μg/mL with recoveries between 92.4 and 103.9 percent. Three plant growth regulators in the concentrated apple juice for quantitative analysis were about 0.15-5.32μg/kg.With the optimization of chromatographic separation column, mobile phase and the wavelength, the HPLC-UV analytical method for four regulators,3-indole butyric acid,4-fluorophenoxyacetic acid, a-naphthaleneacetic acid and 2,4-D has been developed. The retention time of these four regulators were 7.3 min,8.3 min,9.2 min, and 12.0 min, respectively. The detection limits were 0.01μg/mL,0.05μg/mL,0.01μg/mL and 0.05μg/mL with the recovery between 93.6 and 115.3 percent. Four regulators in actual samples were 0.21-5.52μg/kg.The UPLC-MS/MS conditions have been optimized to analyze mepiquat chloride, chlormequat, paclobutrazol and maleic hydrazide by different chromatographic separation columns, mobile phases, detection wavelength, elution mode, ion mode chromatography and mass spectrometry conditions. Within 1.5 minutes four regulators were rapidly baseline separated and their mass spectroscopy character and fragmentation rules ware recommended. The detection limits of the four regulators were 0.6μg/kg,0.7μg/kg,0.8μg/kg and 0.8μg/kg with recoveries between 79.2 and 103.6 percent. Four regulators in actual samples were about 0.83-4.29μg/kg.