Application Of Turboflow Online Purification-liquid Chromatography-tandem Mass Spectrometry In The Detection Of Plant Growth Regulators In Fruits And Vegetables

Plant growth regulator is a kind of material that can regulate plant growth,which belongs to the category of pesticide,and is widely used in agricultural production.Plant growth regulators have certain toxicity,and the food safety problems caused by nonstandard use have seriously threatened people's health and life safety.Turboflow on-line purification technology is a new automatic on-line purification technology,which is fast,efficient and accurate.And connected with liquid chromatography-mass spectrometry can greatly shorten the pre-processing process,reduce the uncertainty factors and improve accuracy.This paper studies the methods by use Turboflow online purification liquid chromatography tandem mass spectrometry to determine IAA,IBA,4-CAP,(2,4)-D,(2,4)-DB,(2,4)-DP,MCPA,MCPP,MCPB,(2,4,5)-T,(2,4,5)-TP 11 kinds of plant growth regulators in bean sprouts and 4-CAP,(2,4)-D,thidiazuron,4-fluoro Phenoxy Acetic Acid,forchlorfenuron 5 kinds of plant growth regulators in grapse.The experiment compared the extraction efficiency of Acetonitrile,ether and ethyl acetate;Comparison of the separation effect of different chromatographic columns Agilient Eclipse XDB-C18(2.1×100 mm,3.5 ?m)and Agilent Eclipse XDB-C18(4.6×150 mm,5 ?m);Optimized the flow phase of Turboflow online purification system and Liquid chromatography analysis system.At last,acetonitrile was used as an extractor.The detection of 11 kinds of plant growth regulators in bean sprouts was gradiently separated by Agilent Eclipse XDB-C18(4.6 x 150 mm,5?m)liquid chromatography column,acetonitrile and 0.1%formic acid aqueous solution as flow phase;Detection of 5 plant growth regulators in grapes with Agilient Eclipse XDB-C18(2.1 x 100 mm,3.5?m)column for gradient separation,methanol and ammonium acetate 5mmol/L+0.1%formic acid aqueous solution as flow phase.The LOQ of 14 kinds of plant growth regulators can reach 10 ?g/kg.The plant growth regulators had a good linear relationship within the concentration range of 10-100 g/L,and the correlation coefficients were greater than 0.99.The recovery range is 70.3%-110%,67.9%-115%;The relative standard deviation is 3.3%-13.3%,4.0%-13.6%.The method established in this study is simple,rapid and accurate,which can meet the requirements of the method for determination 11 plant growth regulators in bean sprouts and 5 plant growth regulators in grapes.