A New Centrifugal Column Chromatography System And The Related Study

Peak asymmetry, which is one of the knotty problems in chromatography study, brings about undesirable effects in both physicochemical studies and application of chromatography. This study aimed to establish an effective method to reduce peak asymmetry by introducing a radial force on the column. The detail is as follows.1. An off-line centrifugal column chromatography system was installed. With this system, the band broadening of two cis-trans-isomers was obtained. Comparing with the classic chromatography, the separating degree in the centrifugal chromatography system was improved. An analysis with the theoretical model showed that the diffusion coefficient was reduced by the centrifugal force in the radial direction.2. An on-line planetary centrifugal column chromatography system was first brought forward and implemented. With this system, the elution profiles of three compounds with low molecular weight were recorded under different rotation speed. The asymmetry parameter and tailing factor were compared and the results implied that the planetary centrifugal column chromatography system could improve peak tailing of elution profiles more compared with the classic column chromatography.3. A physical and mathematical model for the planetary centrifugal column chromatography system was deduced. The theoretical model suggests that total acceleration (α) acting on an arbitrary point in the planetary centrifugal column may cause a radial flow with multi-directions, which could lead to a radial circulation of the mobile phase and the analytes. The circulation could relax the column radial heterogeneity and result in an improvement of peak asymmetry ultimately.