Research Of Quality Standard Of JinShuiLiuJunJian

JinShuiLiu.JunJian come from book of Jingyue Complete Works written by Zhang Jingyue in Ming Dynasty, it is made of Rhizoma Pinelliae.. Pericarpium Citri Reticulatae, Radix Angelicae Sinensis, Radix Glycyrrbizae, Poria, Radix Rehmanniae Preparata and Rhizoma Zingiberis Recens. It mainly be used to cure yin-deficiency of lung and kidney, transmission of dryness and dampness into phlegm, cough, vomiting, nausea, syndrome characterized by dyspnea with phlegm, phlegm with salty taste and so on. Shen Yingsen and Zhao Changying in Medical College of Jinan University had done long-term research of clinic (204 cases) and animal experiment, the result of research indicated that JinShuiLiuiunJian had well therapeutic effect to chronic bronchitis. In order to marketing JinShuiLiuJunJian, its quality standard must be built. This paper researched its quality control method by modem chromatographic and spectrographic instrument. The first, JinShuiLiuiun -Jian and its single medicinal materials were qualitatively identified by thin layer chromatography and thin layer chromatographic scanning through quality index of thin layer chromatogram thin layer scanning chromatogram and ultraviolet scanning spectrogram etc., The second, the content of hesperidin and ferulic acid in JinShuiLiuiunJian were determined by high performance liquid chromatography and the stability and optimize chromatographic determination conditions of ferulic acid were researched. Because hesperidin belongs to flavone compound and that flavone compound had been found in Pericarpium Curl Reticulatae and Radix Rehmanniae Preparata, the content of total flavone compounds in JinShuiLiuJunJian was determined by ultraviolet spect2rophotometry. So the quantitative and qualitative quality standard was built. The last, the hesperidin's animal experiment was researched which was the effectual component of Pericarpium Citri Reticulatae. The result of animal experiment indicated that hesperidin had strong pectroral ability, could improve the content of total phospholipid in I3ALF of big rat of chronic bronchitis model to improve the activity and quantity of pulmonary surfactant,could promote movement and growth of cilium, was the active component of JinShuiLiuJunJian. Those researches proved that our quality standard was effective which primary quality control index was content determination of hesperidin in JinShuiLiuJunJian.