| The resonance scattering spectra of erythrocytes and silver clusters have been obtained. The technique of Resonance Scattering Spectroscopy(RSS) has been applied to the determination of erythrocyte concentration and silver in waste water. silver nanopareticle has been prepared by means of photochemical procedure.The spectra of resonance light scattering, fraction frequency scattering and double frequency scattering for the liquid erythrocyte have been studied firstly. There are fiw peaks of resonance light scattering at 308nm, 468nm, 560nm, 600nm and 936nm. The strongest peak is at468nm. The relationships among resonance light scattering, fraction frequency scattering and double frequency scattering have been investigated. The nonlinear scattering mechanism has been discussed. The results show erythrocyte is a kind of nonlinear scattering medium. The half-width of peak, the ratio of half-width of peak and the ratio of height of peak are not related to erythrocyte concentration, and the half-width of peak is linear to the wavelength of peak. The quantitative relationships between the 'RSS and the concentration of the liquid erythrocyte have been established. With A ex468nm,within the concentration region from 0.015x106 ImL to 31.1x106 ImL , it has a regression equation:A I=17.002C+3.870, its linear coefficient is 0.9992 and detection limit is 0.015x106 /mL, I~ is stable within lh. According to aforesaid, a new method is proposed to determine erythrocyte concentration. The influence of Cr3~ and wraped Au colloid on erythrocyte has been investigated. The3results show that Cr~ make 'RLS of erythrocyte reduce. It may be due to the Cr3~ being combined with the carboxyl in erythrocyte membrane. The wraped Au colloid make the strongest peak of erythrocyte move to 600nm.In the medium of sodium chloride and sodium citrate ,the Ag clusters have been prepared by microwave highpressure liquid synthesis. These Ag clusters are stable and its diameter is about 10 nm. With A ~~=425nm,it exhibits the strongest resonance scattering peak at 425nm and a 1/2- fraction- frequency scattering peak at 850nm. With A ex=6OO~im,there are a 2-double frequency scattering peak at 300nm and a 2/3 -fraction frequency scattering peak at 900nm. With A ex8SOnm, it exhibits a 3-double frequency scattering peak at 283nm, a 2- double frequency scattering peak at 425nm and a 2/3- fraction frequency scattering peak at 567nm. The fraction frequency scattering peak, the double frequency scattering peak and the resonance scattering peak have similar scattering property. Various kinds of scattering spectra for liquid silver clusters have been explained by the principle of fraction frequency, sum frequency and differential frequency.In the medium of 0.O4mol/L H-~SO4 and 0.3%tritonX-10, Ag(I)was reduced photo chemically to form silver collide by means of UVlight. It exhibits a sensitive resonance scattering peak at 450nm. A new photochemical resonance scattenng spectrophotometric method for 0.1-4 vi gImL Ag has been described. It has a regression equation:~ I=12.6C+0.26 and a detection limit of 0.05 vi gImL Ag. The results show that this method has rapidity and easy to operate when it is applied to the determination of silver in waste water.
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