Solid-phase Chemiluminescence System

SOLID-PHASE CHEMILUMINESCENCE SYSTEMZhao lixiaAbstract: Two parts, review and research report, are included in this thesis.In the first part, some reagents required for reactions that produce luminescence as labels has been reviewed. Because the methods based on fluorescence, chemiluminescence and bioluminescence have very low detects, they have the potential to replace assays that employ radioisotopes as labels. This review includes the labeling principle, detection methods and characteristics of some luminescence labels.In the second part, the three solid-phase chemiluminescence system were studied and applied to the drugs analysis.1. A chemiluminescence assay for the determination of Pipernidic Acid using NaBiOs solid-phase reactorIn this paper, a novel solid-phase reactor for Pipernidic Acid was prepared by adsorption of sponge in a flow injection system. It was applied to the determination of Pipernidic Acid based on the chemiluminescence reaction of NaBiO3-Na2SO3-Pipernidic Acid. Under optimum conditions, The linear range for the determination of Pipernidic Acid was 1 x 10"7g/ml~1.0 * 10"5g/ml, the detection limit was 6.2 * 10'8g/ml, the relative standard deviation were 2.5% for 2 * lO^g/ml Pipernidic Acid (n=9).The method has been applied successfully to the determination of Pipernidic Acid in medicine. The solid-phase reactor is stable for over 400 determinations2. Flow-injection chemiluminescence assay for OFLX using PbO: solid-phase reactorA novel solid-phase reactor for OFLX was prepared by adsorption of sponge and in a flow injection system. It was applied to the determination of OFLX based on the chemiluminescence reaction of Pb02-Na2SOs- OFLX. Under optimum conditions, The linear range for the determination of OFLX, were 2 * 10'7g/ml~1.0 * \0'5g/m\, the detection limit was 7.8 x lO^g/ml, the relative standard deviation were 1.6% for 2 x lO^g/ml OFLX(n=9).The method has been applied successfully to the determination of OFLX in medicine. The solid-phase reactor is stable for over 400 determinations3. Mn(>2 chemiluminescence system(1) Flow-injection chemiluminescence assay for quinine using MnOj solid-phase reactorA novel solid-phase reactor for quinine was prepared by adsorption of sponge and in a flow injection system. It was applied to the determination of quinine based on the chemiluminescence reaction of MnO2-Na2SO3-quinine. Under optimum conditions, The linear range for the determination of quinine was 4.0 x 10~7M~8.0 * 10"5M, the detection limit was 3.3 * 10~7M, the relative standard deviation were 2.1% for 1 x lOg/ml quinine (n=ll).The method has been applied successfully to the determination of quinine in medicine. The solid-phase reactor can be reused over 400 times. (2) Flow-injection system for automated dissolution testing of anaglin tablets with chemiluminescence dectionA simple and sensitive flow-injection chemilumiescence(CL) system for automated dissolution testing is described and evaluated for monitoring of dissolution profiles of analgin tablets. The undissolved suspended particles in the dissolved solution were eliminated via on-line filter. The novel CL system of MnCV analgin -RhB was also investigated. A novel solid-phase reactor was prepared by adsorption of sponge. The sampling frequency of the system was 60h'1. The dissolution profiles of analgin release tablets from three sources were determined, which demonstrates the stability, great sensitivity, large dynamic measuring range and robustness of the system.