Investigation On The New Chemiluminescence Analytical Systems And New Chemiluminescence Analytical Methods

Chemiluminescence (CL) analysis has attracted people's attentions in recent years for the analysis of a great number of important inorganic and organic species in different fields such as biotechnology, pharmacology, molecular biology, clinical medicine and environmental detection mainly owing to its attracting features including low detection limit and wide linear dynamic range, both of which usually can be achieved with simple and relatively inexpensive instrumentation. Current research in CL analysis is focused on two general directions. One direction is the discovery of new CL reactions to broaden the analytical scope of CL analysis and the other direction is the combination of the development of CL detection systems with newly developing techniques, such as capillary electrophoresis to improve the selectivity of CL analysis and increase the applied value of CL method.This investigation is dedicated to two purposes. One purpose is to improve the selectivity of the CL method by the combination of CL detection system with molecularly imprinted technique or controlled-potential electrolytic technique. The other purpose is to find new CL reactions, establish new CL methods and study their applications to real samples.Chapter 1 General introductionIn this chapter, the CL basic principle, the CL instrumentation, the typical CL reactions and their analytical applications in the past three years were summarized. Thenewly developing techniques and the future trends were also outlined.Chapter 2 Molecular imprinting-chemiluminescence analysisMolecular imprinting technique is a rapidly developing technique for the preparation of polymers that possessing specific recognition function for interesting molecule. The attractive features of MIP, such as mechanical strength, ease of preparation and stability in harsh environments make it to be used as chromatographic stationary phase, solid phase extraction matrices, artificial receptors for use in drug assays and recognition elements in sensors. In this chapter, two novel flow-through CL detection systems were designed for the determination of epinephrine and paracetamol by using epinephrine-imprinted polymer and paracetamol-imprinted polymer as molecular recognition material, respectively. The methods are based on the direct CL reaction of epinephrine with luminol in the presence of potassium ferricyanide and potassium ferrocyanide in alkaline solution or based on the inhibitory effect of paracetamol on the luminol-potassium ferricyanide CL reaction. The molecularly imprinted polymers were prepared by using methacrylic acid (for epinephrine) or acrylamide (for paracetamol) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker in the presence of template molecule: epinephrine or paracetamol. A portion of resultant polymer with particle size of 100-200m was packed into a flow cell, which was placed in front of the window of the photomultiplier tube. While epinephrine or paracetamol solution flowing through the flow cell, epinephrine or paracetamol molecule was selectively adsorbed by molecularly imprinted polymer packed in the flow cell. Afterward, the CL reagents flowed through the flow cell; reacted with epinephrine or paracetamol molecule adsorbed in the molecularly imprinted polymer and produce CL emission. During the CL reactions, epinephrine or paracetamol molecules were destroyed leaving the cavities for next determination. The CL intensity responded linearly to the logarithm of the concentration of epinephrine within 5.0+10-9-1.0+10-7 mol/Lrange. The detectionSouthwest Normal University, Doctor Thesis ABSTRACTlimit was 3+10-9 mol/L and the relative standard deviation was less than 5% (2.0+10-8 mol/L epinephrine solution, n=7). And for paracetamol, the decreased CL intensity responded linearly with the logarithm of the concentration of paracetamol over 7-100mol/L range. The detection limit for...