Studies On Immobilization Of Chloroplast In PVA-SbQ And Its Application In Herbicide Detection

Polyvinylalcohol bearing styrylpyridinium group is a new photo-induced cross-linking polymer. Ultraviolet can induced it to form network. Ultra-thin section photographs showed the intactness and orderly arrangement of the inner and outer membranes of the thylakoid entrapped in PVA-SbQ, after stored under -20 C for half a year. The activity of the membrane remained over 50%.Chloroplast has the ability to defense outer and inner stresses because it's inner enzymes system can scavenge superoxygen radical and hydrogen peroxide. And herbicides can affect this ability. Stick the immobilized membrane to Pt electrode surface. Using a differential pause voltammetry method working in 50mM Tris-HCl (pH 7.4) buffer(with 0.03 % H202) under 25*C, the concentration of working curves of paraquat, diuron,ametryn,prometryn and atrazine ranged 0.0014-0.028, 0.019-0.19, 0.12-1.20, 0.20-1.0, 0.24-1.20 ppm respectively.This chloroplast biosensor can be used not only to detect herbicides, heavy metal ion and pesticides, but to evaluate the quality of drinking water.