Study On Culture And Preparation Of Lactic Acid Bacteria Powder Starter Used For Fermented Meat

In order to get lactic acid bacteria powder starter with a largo number of live cells, the medium, cutture conditions, carrier and protecting agent for powder starter were studied in this experiment. The results have indicated that the composition of the medium for Lactobacillus fermentum and Lactobacillus plantrum is 2% sucrose, 0. 5% peptone and 11% soybean powder. The optimum inoculum size is 3%, optimum temperature is at 38.0C .Large number of lactic acid bacteria cells can be gained in shorter time with 0.5% KH2PO4-Na2HPO4 added. The selected medium inoculated with 3% Lactobacillus fermentum and Lactobacillus plantrum are cultivated at 38.0 C for 18 h, and the cell concentrations can reach 8. 72 X 109 and 3. 01 X 109 per milliliter medium, respectively. The mixture of 50% non-fat milk power and 50% starch is the more effective and cheaper carrier. The concentration of Lactobacillus fermentum and Lactobacillus plantrum is 1. 21 X 1010 cfu/g , 9. 84 X 109 cfu/g in powder starter with glycerol added and the cell survival is still 65.2% and 69. 1% .respectively, after 90 days storage. The sausages inoculated with liquid starter and powder starter were processed and there is no obvious difference in sensory and in acid production during fermenting.