| The best process route of extraction on Pholiota nemako polysaccharide (PNP) and its anti-tumor activity on tumorigenic BALB/c mouse were firstly studied. Three results showed as follow:1. An united method, that was extraction in water after enzymolysis (cellulase and trypsin), was used in this study. And the best extraction route was showed following: the proportion of digestion 1:10, cellulase enzymolysis (0.05% dosage, pH 5.0, 45 癈) sixty minutes, trypsin enzymolysis (0.05% dosage, pH 8.0, 25 癈 ) fourty-five minutes, then extraction keeping warm and stirring ninty minutes. The study result showed that the extractive efficiency was not as good as prolonging extractive time in the stage of hot water-extraction after enzymolysis. That was, the efficiency will decrease after some time. The extractive efficiency of this united method was better than the pure enzymolysis and pure extraction in hot water with promotion of 37% and 66% respectively. The efficiency was notable and polysaccharide extracted by this method was easy to be purified. All these showed that the extractive route was practicable and easy.2. PNP extracted by this united method was dust-coloured and neutral polysaccharide and were composed of D-mannose, D-glucose and D-galactose. PNP was a homogeneous ingredient after deproteinization by sevag-method and graded by ultracentrifugation. Its sugar content was up to 9 1 . 1 %.3. The animal experiment results showed that: PNP could promote the livability of tumorigenic mouse; could accelerate the growth and differentiation and put on weight of spleen; could increase the content of TNF- a in normal mouse' serum and decrease the content of tumorigenic mouse by different pathways probably; and could decrease the content of IL-6 in tumorigenic mouse' serum, but had no effection on normal mouse. In addition, PNP could inhibite the growth of tumor by decrease the activity of LDH in tumor-organization.In all, PNP could enhance the imrnunity-systerm of tumorigentic or normal mouses.
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