Synthesis, Characterization And Applications Of Fluorescent Reagents Of 9-Aminoacridine Derivatives

A novel synthetic method of 9,9' -ethyldiaminodiacridine(AI), 9,9' -propyldiaminodiacridine(A2) and 9,9' -triethylenetraaminodiacridine(A3 ) as potential antitumor was reported; three new reagents: 9-(2-aminoanili o)acridine(B1), 9-(3-aminoanilino)acridine(B2), 9-(4-aminoanilino)acridine (B3), have been synthesized ; and two known compounds: 9- -hydroxyl ethylaminoacridine(Ci), 9-aminoacridine(C2), have also been synthesized. IR, 'HNMR and elemental analysis have characterized compounds. We tried to synthesize 9-carboxymethylaminoacridine(C3), 9-diethanolamino acridine(C4) and 9-triethanolaminoacridine(C5), to discuss and analyze the results. Besides, 9-methylacridine was synthesized by microwave radiation; this method was simple and convenient.The fluorescent enhancement of 9-(2-aminoanilino)acridine(Bi, o-APAA) by Chromium(VI) in the sulfuric acid medium was studied, so a method of fluoremetry for the determination of trace Chromium(VI) was established. A fluorescence enhancing method based on the studies of interaction of o-APAA and Bovine serum albumin(BSA) for the determination of BSA was studied, the result indicated that this method had the advantages of high sensitivity, good selectivity and simplicity. The fluorescence spectra of 9-(3-aminoanilino)acridine(B2,m-APAA) with Deoxyribonucleic acid(DNA) has been studied, so a fluorescent method for the determination of DNA was established; this method had the advantages of high sensitivity, good selectivity and simplicity.