| Chemiluminescence (CL) is defined as the emission of light from an electronically excited state species which is produced during the course of a chemical reaction, and is observed when the electronically excited product or intermediate formed decays to the ground state with a photon emitted. Due to its high sensitivity, wide linear range, rapidity, simplicity and relatively low cost, chemiluminescence analysis has been widely applied in environmental science and life science and has become one of the most powerful technique in analytical chemistry.The thesis consists of two parts. The first is a review dealing with the application of chemiluminescence in environmental and pharmaceutical analysis. Emphasis is placed on the development in the rencent ten years.The other is research reports which is composed of 6 components as follows. 1) Luminol-hexacyanoferrate (ni)-phenol chemiluminescence system; 2) Luminol-potassium permanganate -phenol chemiluminescence system; 3) Luminol-hydrogen dioxide-nitrite chemiluminescence system; 4) Determination of ergonovine maleate using fluorescence detection; 5) Flow injection chemiluminescence sensor for the determination of ergonovine maleate; 6) Study on the drug concentration-time curve of ergonovine maleate by microdialysis-flow injection-flourescence system.1. Luminol-Hexacyanoferrate (III) -Phenol Chemiluminescence SystemA new chemiluminescence method for the determination of phenol based on its sensitization on the Luminol-K3Fe(CN)6 system was presented. The linear range for phenol is 0.01-10ug/mL with a detection limit of 4X 10-3ug/mL, and the relative standard deviation forlug/mL phenol is 1.06%(n=ll).The proposed method has been applied to Ihe determination of phenol in syntherical and industrial waters with satisfied results.2. Luminol-Potassium permanganate-Phenol Chemiluminescence SystemA new chemiluminescence method for the determination of phenol based on its sensitization on the Luminol-KMnO4 system was presented. The linear range for phenol is 0.001 -2.5ug/mL with a detection limit of 3.5 X 10ug/mL, and the relative standard deviation for 0.05ug/mL and 0.5ug/mL phenol is 0.6% and 2.5%, respectively (n=ll). The proposed method has been applied to the determination of phenol in syntherical and industrial waters with satisfied results.3. Luminol-Hydrogen dioxide-Nitrite Chemiluminescence SystemA new chemiluminescence method for the simultaneous determination of nitrite and nitrate was presented. Nitrate was on-line reduced to nitrite by spongiform zinc-cadmium reducer, and nitrite was determined based on the chemiluminescence emission produced upon mixing alkaline luminol solution with peroxynitrous acid that was created when nitrite reacted with hydrogen dioxide solution in acid medium. The system responds linearly in the range of 3.3 X 10-3.3 X 10mL for nitrite and 1X10-6-1 X 10-4mL for nitrate. The detection limits for nitrite and nitrate were 1.6X 10g/mL and 3.3 X I0g/mL, respectively.This method has been applied to the analysis of natural waters and syntherical samples with satisfactory results.4. Determination of ergonovine maleate using fluorescence detectionA novel fluorescence method for determination of ergonovine maleate based on its inherent fluorescence in aqua solution was proposed with advantages of simple, rapid and senstitive. The exitation and emission wavelength were set at 325nm and 43 Inm, respectively. The linear range for ergonovine maleate isl X 10-4- 0.2}g/mL with a detection limit of 4X 10-5ug/mL, and the relative standard deviation for 0.05ug/mL ergonovine maleate is 0.32% (n=ll). The proposed method has been applied to the determination of ergonovine maleate in injection and plasma.5. Flow injection chemiluminescence sensor for the determination of ergonovine maleateA novel flow injection chemiluminescence (CL) sensor for the determination of ergonovine maleate has been proposed. Hexacyanoferrate(ni) was immobilized electrostatically on an anion-exchange resin column and was eluted from the colu... |
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