Study On Resolution Of Racemic 2-methyl-1-butanol Catalyzed By Lipase

Lipase have great potential as catalysts for use in organic synthesis, because of their high catalytic efficiency and stereoselectivity. As techniques for lowering lipase cost available, lipase catalyzed organic reactions are of great value as a new synthesis method. Enzymatic catalysis in organic solvent is one of the most interesting topics. Because of the importance of chiral drugs, considerable efforts have been made in developing bio-transformation for the production of chiral drugs.Some important alcohols are synthesized through transesterification processes using an ester and an alcohol. In this paper, efficient resolution of a racemic 2-methyl-1-butanol was achieved by Candida cylindracea Lipase (CCL) and Porcine Pancreatic Lipase (PPL) catalysed transesterification in non-aqueous organic media. To overcome the problems in using free lipases, immobilized lipases have been used as catalysts on supports. To find the optimum factors such as PH , temperature ,the amount of lipase, the different supports and different immobilized methods, CCL and PPL were immobilized on kinds of supports by different methods . The dispersion of lipase in organic solvent was improved effectively by means of adsorbing lipase on GDX-102. It was found that the immobilized lipase in organic media remained a very high activity.The free and immobilized lipase were catalyzed the transesterification of 2-methyl-l-butanol and triburyrin. The influences of organic solvent , water content , ratio of substrates , immobilized lipase content , reaction time and temperature on resolution were systematic studied. In experimentconditions, thermal stability and operation stability of the immobilized lipase were more excellent than those of the free lipase. Experimental observations indicated that the lipase can be reused. The optical purities of productds can over 98%.