Studies About The Interactions Between The Analytical Reagents And The Biomacromolecules

Alizarin red (AR) and Acid chrome blue K (ACBK) are supposed as probes of protein. Phenosafranine (PS) is supposed as probe of nucleic acid. All of them are analytical reagents. The interactions between the analytical reagents and the biomacromolecules have been studied. It is showed that:1. The interaction of alizarin red (AR) and bovine serum albumin (BSA) is investigated in the buffer solutions at pH2.68. It is considered that the combination of AR and BSA is due to the electrostatic force and the "Phase Distribution Model" is the proper description of the interaction between AR and BSA. According to the assumption of Pesavento, the distribution ratio D of AR between "protein phase" and "water phase" is obtained. It is studied that the effection of sodium dodecyl sulphate (SDS) at different concentrations on the interaction between AR and BSA is mainly due to the electrostatic force and hydrophobic force by discussing the relate parameters such as A, D, K , AG, AH and AS.2. Spectrophotometric characters of the interaction between the protein and Acid chrome blue K (ACBK) have been studied in acidic and basic solutions. The interaction mechanism of BSA and ACBK in different microenvironment because of the change of pH values has been discussed tentatively. Protein can combine with ACBK to reduce the absorptivity of ACBK at pH 3.78 and make the A max reduce 31nm but make the A max reduce 20nm at pH9.91. The interaction is studied by using two methods, Spectrophotometric method and equilibrium dialysis method, with relevant comparison. It is proved that the interaction is in accord with model of phase distribution. Moreover, according to the calculations of A, D, K, A G, AH, AS and the configurations of ACBK and BSA at different pH conditions, the interaction between BSA and AR is mainly due to the electrostatic force at pH3.78 and hydrophobic force at pH9.91.Ill3. The interaction of phenosafranine (PS) with DNA has been studied in theBritton-Robinson (B-R) buffer solution at pHV.OO on the naked silver electrode by cyclic voltammetry. Adding DNA into the PS solution resulted in the decrease of peak current without the change of equilibrium peak potential, which indicated that an electrochemical inactive complex was formed. The ipa of PS decreased proportionally to the DNA concentrations from 0.50 to 8.00mg/L. Moreover, the interaction mechanism of PS and DNA has been discussed tentatively.