Studies On Methionine-Chromium(Ⅲ) Chelate Using As Food Additive

Chromium(Chromuim,Cr3+) was one of the microelements necessary for human body and the component of alive center of series of enzymes and protein. Chromium made a very important role in maintaining matter metabolism and physiological function in human body. It could not only eliminate free radical but also restrain the production of free radical. Methionine was an essential amino acid in human body, it possessed some biological activity and was natural Hgand of chromium. Methionine could also eliminate free radical effectively. Methionine was combined with chromium for their special absorption and transport in body, which strengthened their biological function. Chromium-Methionine chelate was a new nutritional intensifying agent.The Chromium-Methionine chelate was synthesized by methionine and chromium chloride. The final reactant was washed by water and ethanol, and then we got the Chromium-Methionine chelate.The optimum conditions were that molar ratio of methionine to chromium-chloride was 3:1, reaction temperature was 80癈, reaction pH was 7.0, reaction time was 60 min and the concentration of methionine was 15 percent. The yield was 48.41%. Composition and structure of the complex were characterized by amino acid analysis, elementary analysis, UV spectrogram analysis, infrared spectrum and mass spectrum analysis. Chromium-Methionine's molecule composition was [Cr(NH2CHCH2CH2SCH3COO)3 ] . Relatively molecule mass was 497.0.The melting point of Chromium-Methionine chelate was 352-356 .The solubility of Chromium-Methionine chelate was 42.00mg/100g H2O.The security of complex was appraised by animal experiment. The results made clear that Chromium-Methionine chelate was safety. Milk powder was added with different concentration of complex at the same time. By determining some nutrition index of milk powder such as fat, VE, VB2, VB6, VPp and so on, we could make sure the complex destroied VB2, VB6 and VE, but it didn't destroy VPP and fat. If we ignored effects on VB2, VB6 and VE, we could use Chromium-Methionine chelate as additive for milk power of diabetic.