Methodology Studies Of Echinacea And Eucommia Ulmoides Et Al By LC/MS And Preparations Of Related Standards

With the emergence of atmospheric pressure ionization (API), combined high performance liquid chromatography and mass spectrometry (LC/MS) technique has been rapidly developed in past two decades. It takes advantages of chromatography as a high performance separation method and of both photodiode array detection (DAD) and MS as a sensitive identification method. Its applications have been extended to phytochemistry, analytical chemistry, biochemistry, clinical and pharmacological researches and so on.The studies in this thesis are summarized as follows:1. An HPLC-DAD-ESIMS method was developed for the simultaneous analysis of caffeic acid derivatives and alkamides in the roots and extracts of Echinacea purpurea. The method is better than previously published techniques in product quality control, and can provide HPLC fingerprints of bio-active components in Echinacea purpurea.2. An HPLC-DAD method for the determination of cichoric acid in Echinacea purpurea was reported by use of 400 mmol/L ammonium acetate-methanol (95:5) as mobile phase. The method was simple, sensitive, accurate and precise, and had been successfully applied in the determination of cichoric acid in Echinacea purpurea. L. extract and Echinacea purpurea. L. preparations.3. A rapid, specific and reproducible HPLC-DAD- ESIMS technique was developed for the determination of nine bioactive compounds in Eucommia ulmoides Oliv. The newly developed technique has been used for the rapidanalysis of a number of samples includingEucommia ulmoides cortex, leaves, extracts, mixed preparations of traditional Chinese patent medicines and beverages. This method can be used for quality control of medicinal materials of Eucommia ulmoides Oliv and its preparations.4. An HPLC/APCIMS method for separation and determination of alkaloids in Macleaya cordata (Willd.) R. Br. was established. HPLC/APCIMS fingerprints of alkaloids in Macleaya cordata (Willd.) R. Br. were obtained.5. Purification and separation of cichoric acid, panaxtriols and panaxadiols by preparative HPLC were discussed.