| Chemiluminescence(CL) is defined as the emission of light from an electronically excited state species which is produced during the course of a chemical reaction, and is observed when the electronically excited product or intermediate formed decays to the ground state with a photon emitted. There are many merits on CL such as high sensitivity, wide linear range, rapidity, simplicity and so on. Due to the power ability of seperation, high performance liquid chromatography coupled with chemiluminescene has been widely applied in analysis on complicated, biologic samples and has become the most powerful technique in analytical chemistry.The thesis consists two parts. The first part is a review concering the definiton, the classification and the analytical methods of neurotransmitters. Emphasis was placed on the development of technique of high performance liquid chromatography coupled with chemiluminescene detection in the recent ten years.The other is research reports which is composed of 6 parts as follows. There are two chemiluminescence systems was described concluding Luminol-K.3Fe(CN)6 and luminol-H2O2 systems. Neurotransmitters were separated and determined by HPLC-CL, and satisfied results were obtained.1.Chemiluminescence Determination of Dobutamine Hydrochloride in Human Serum by HPLC Separation coupled with Ultrafiltration TechnologyA novel chemiluminescence determination of dobutamine hydrochloride in human serum by HPLC separation connected with ultrafiltration technology is described in this paper. The signal produced by the chemiluminescent reaction(CL) between luminol and ferricyanide was greatly increased in the presence of dobutamine which was eluted by HPLC. The linear range of dobutamine concentration was 1.0 X 10-9gmL-1 - 1.0 X 10-7gmL-1 (r=0.9995)and the detection limit was 7.6X 10-10gmL-1.2.Determination of Dopamine Hydrochloride in Human Serum and Urine Samples by High Performance Liquid Chromatography with Chemiluminescence DetectionThe separation and determination of dopamine hydrochloride in human serum and urine samples by high performance liquid chromatography with Luminol-K3Fe(CN)6 chemiluminescence detection were investigated first time in this paper.The separation was carried out on C18 colum using the moble phase of 0.01mol/L potassium hydrogen phthalste solution and methyl alcohol(V1:V2=92%:8%).Under the optimum conditions of 2 X 10-4mol/L Luminol(NaHCO3/NaOH solution ,pH=9.8), 2X10-5mol/L K3Fe(CN)6,the linear range and detection limit for dopamine hydrochloride were 5~1000ng/mL and 8.0 X 10-10g/mL, respectively. The relative standard derivation for 100ng/mL dopamine hydrochloride was 3.2%(n=11).3.Chemiluminescence Determination of Levodopa in Human Serum and Urine SaBipies by HPLC SeparationA flow injection chemiluminescence method for the determination of levodopa in human serum by HPLC separation connected with ultrafitration was described in this paper.The method is based on the chemiluminescence produced during the oxidation of luminol by potassium ferricyanide in an alkaline solution.The emission intensity is greatly enhanced in the presense of levodopa which was eluted by potassium hydrogen phthalate solution.Under the optimal conditions ,the CL intensity was linear to the concentration in the range of 1 .0X 10-8g/mL-1.0 X 10-6g/mL(r=0.9995),With a detection limit of 3.0 X 10-9g/mL.Relative standard deviation was 2.8%.The proposed method was simple,quick and sensitive. 4. Determination of Noradrenaline Bitartrate in Human Serum by HPLC Separatien with Chemiluminescence DetectionThe separation and determination of noradrenaline nitartrate in human serum by high performance liquid chromatography with Luminol-K.3Fe(CN)6 chemiluminescence detection were investigated first time in this paper.The separation was carried out on C18 colum using the moble phase of 0.01mol/L potassium hydrogen phthalate solution and methyl alcohol(V1:V2=92%:8%).Under the optimum conditions of 2 X 10-4mol/L Luminol (NaHCO3/NaOH solution ,pH=9.8), 3X 10-5mol/L K3Fe(CN)6,the...
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