The Preliminary Study On Polysaccharide From Pyracantha Fortuneana

Abstract Pyracantha fortuneana (Maxim.) Li, which was also named Chiyangzi and Jiujunliang, is mainly distributed in the southeast and southwest of China. Its fruit, leaf and root all could be used as medicine in China. Furthermore,its fruit was edible, which contained abundant nutriment. So Pyracantha fortuneana (Maxim.) Li fruit had great exploitation value.In order to ulteriorly exploit Pyracantha fortuneana (Maxim.) Li resource, the author conducted a series of study on the water-soluble polysaccharide from Pyracantha fortuneana and achieved the following results.The optimal extraction technology of Pyracantha fortuneana polysaccharides were 90℃, 9h, 10:1 (WAV) of water to its fruit. Extraction ratio of the polysaccharides was about 3.5%. With ultrasonic, extraction ratio can be increased more than 30%.Through antioxidant in vio experiment by DPPH . spectrophotophy, it showed that Pyracantha fortuneana crude polysaccharide could remove DPPH . and its effect was very prominent and SC50 was 1.87mg/mL.Some experiments indicated that the protein could be almost clearly removed from Pyracantha fortuneana crude polysaccharide by protein-enzyme-Sevag and decolor could be achieved by LSA-8 big size adsorption resin. Then isolated by DE-52 and Sephadex G-200 gel filtration, three glycoconjugates PP-A2,PP-A3 and PP-B2 were attained.Gas chromatography analysis, infra-red spectra analysis and physical and chemical characters showed that PP-A2 and PP-B2 were the same materials. They mainly contained rhamnose, arabinose, fructose, galactose and mannose in a ratio of 5.05:4.51:3.37:1.18:1. Sephadex G-200 gel filtration analysis showed that their molecular weight was 30,000dal. Different to PP-A2, PP-B2 contained a little pigment. PP-A3 was a kind of glycoprotein. It was composed of arabinose, glucose and fructose in a ratio of 1.62:1:1.59 and some amino acids whose total content was 0.5%. In amino acid, cysteine was not detected out. The molecular weight of PP-A3 was 210,000dal.As dietary fiber functional food additivies, Pyracantha fortuneana polysaccharide has good expansibility, ability to keep water and ability to exchange with positive ion. Furthermore, it can scavenge free radical. When it is used as meliorative additive in noodle, the dosage of Pyracantha fortuneana polysaccharide is about 3%.