Preparation Of Chromium With Collagen Complex And Its Function Of Lowering Blood Sugar

In this thesis, the hydrochloric acid assimilation solution and 0.2M CrCl3 were mixed in a certain proportion, which could make Cr3+ and peptide or amino acid in the assimilation solution chelate. The chromium content was determined in chemical titration and the chelating extent was determined in infrared spectrum. The substance chelating chromium was treated as a functional factor lowering blood sugar, whose influence to the blood parameter and liver of diabetes rats starch was determined.Experimental animals are KM rats, by injecting alloxan in the dose of 200mg/kg into the abdomen, diabetes animal model were established, in comparison, normal rats were injected in the same dose of 0.9% sodium chloride solution. After this, those were treated as diabetes model rats whose fasting blood sugar content was above 11.1mol/L. Experimental rats were divided into 5 groups: normal rats, normal rats fed chromium, diabetes rats, diabetes rats fed chromium, diabetes rats fed chromium and rice bran. Diabetes were raised in the dose of 1ug/d Cr3+ by drinking, 6 subject rats in each group were killed every week, their blood parameters were determined by biochemical reagent kit. After 6 weeks administration, no significant difference can be observed between normal rats blood parameters and those of rats fed chromium(P>0.05); there was an obvious decrease between the blood sugar content and triglyceride of diabetes rats and those of diabetes raised chromium(P<0.05); rice bran and chromium did have no effect on the blood sugar and other parameters, the former changed violently and had no difference in comparison with diabetes rats(P>0.05). The livers were taken from each group randomly to homogenize them, the soluble protein was extracted in the 0.9% sodium chloride solution and deposited in alcohol. After centrifugation, the protein was dissolved in the sample buffer solution and separated in SDS-PAGE, there was no difference between the protein strips.70 rats were divided into 2 groups, one was fed chromium in the same method as above in comparison with the other, 6 rats each group were killed and blood parameters were determined in the same method.. After 12 days administration, there was no difference between normal rats and those fed chromium, though emerging significant difference at the beginning, their livers were disposed in the same method as above, the result showed obvious difference.Heat stress experiment: 24 rats were divided into 4 groups, of which the half is male and the other female. One male group and female group were fed chromium in the dose of lug/d, after 4 weeks administration, all rats were placed in the oven at 43 C with relative humidity, the heat tolerance time and the death rate after 1hour of each rat was noted. The heat tolerance time of female rat was longer than that of male rat, but there was no significant difference between those. The heat tolerance time of female rat fed chromium was more longer than the other female group(P<0.05), and there was no difference between the male groups(P<0.05), and the soluble protein extracted in the 0.9% sodium chloride solution was separated in SDS-PAGE.