Application And Study Of Resonance Light Scattering Turbidimetry In Environmental Analysis

On the basis of Resonance light scattering technique and Turbidimetry, this paper advances a new spectral analytical method of combining the two of them-Resonance Light Scattering Turbidimetry, that is RLST. And, the paper also gives the research about the theory of RLST as well as its application in the environmental analysis.there is a brief introduction of the researchful actuality and history about Resonance Light Scattering Technology and a conclusion about the characteristics of this technology. Besides, this part also reports that it's developing tendency for this technology to reenforce the fundamental theoretical study and enlarge the applicative fields of Resonance Light Scattering. We also reports two other dyes as probes of RLS in determining protein.There is to introduce the basic method and theory of Turbidimetry. It draws a conclusion on the application actuality, advantages and disadvantages about Turbidimetry which is used in environmental analysis, clinical analysis, industrial analysis and so on.Based on the combination of the characteristics of Turbidimetry, the basic theory of Resonance Light Scattering Technology and the colloid chemistry, we put forward Resonance Light Scattering Turbidemetry, which uses Resonance Light Scattering Technique as the test technique of the new spectral analytical method. We say that the formation of the big stable particulate colloid system is the basic qualification for RLST. And the rising and falling phenomena of colloid solution is one of the reasons for the light scattering. This paper forms the ration equation of RLST. At the same time, we prospect of the application foreground of RLST.we study the application of RLST used in the determination for Chloride ions, Sulfate ions, and Potassium ions. In the study of determining Chloride ions withRLST, it is found that the Resonance Light Scattering intensity of the blank is weak. However, when it becomes silver chloride colloid liquid, the Resonance light scattering increases obviously. At the place where A =510nm, there exists a strong and stable light scattering intensity. Within 0~14ug/ml, Resonance light scattering intensity has the linear relationship with the thickness of Chloride ions. The detection limit is at 0.04ug/mL. The recoveries of the method are in the range 98.7%~102%. The relative standard deviation is between 1.1%~2.2%. Using this method to determine the Chloride ions in the tap water and Chloride ions in acid copper plating bath, we can get a satisfactory result. During the study of determining the sulfate ions with this method, we also can find the Resonance light scattering of the blank is weak. But after we add the Sulfate ions, the Resonance light scattering intensity will remarkably increase. And it will show us the linear relationship within 2~25ug/ml, with the detection limit at 0.23ug/ml. Testing the sulfate ions in the salt with this method, it proves that the method can be used in the practical production. What's more, we establish a system for determining potassium ions with RLST. In the B-R buffering solution, the reaction of Potassium and sodium tetraphenyl borate can form a scattering system which will greatly increase the intensity of Resonance light scattering. At the place where X =578nm,the light scattering is the maximum and stable, with 0.0-4.6 X 10 ~4mol/L, it has the linear relationship with the thickness of Potassium ions. The detection limit is at 0.124 X 10~6 mol/L. With this method to determine the content of Potassium ions in the Urine, we can get a result that satisfies us. We have also studied the best conditions for the three experimental systems and the interference situation of the coexistent ions. The result of the experiment proves that all of the three systems have the same characteristics: high sensibility, simpleness and high speed.