Flow Injection Chemiluminescence Analysis Of Antibiotics Based On N-bromosuccinimide

Luminescence is one of the phenomena which was reported in Greece civilization age from excited state molecule decays to the ground state with a photon emitted. Although the first "artificial"chemiluminescent phenomenon was reported by Radziszewski in 1887, studies and cognition on the chemiluminescence was slow from the luminescent phenomena to the research of the luminescent reagent and system, from the unite of the chemiluminescence technique to the improvement of the chemiluminescence instrument. Chemiluminescence (CL) is defined as the emission of light from an electronically excited state species which is produced during the course of a chemical reaction, and is observed when the electronically excited product or intermediate formed decays to the ground state with a photon emitted. Due to the simplicity, low detection limit, large calibration ranges and rapid analysis speed, chemiluminescence analysis has been widely used in practical applications and pharmaceutical analysis.Antibiotics is the drugs which can kill bacterium , fungicidal virus and chlamydia. It become the main drugs which have been widely applied to the remedy of the poultry, crop and disease. Due to the simplicity, rapid analysis speed, chemiluminescence analysis has been widely used in antibiotics.The thesis consists of two parts. The first is a review dealing with the application of chemiluminescence in pharmaceutical analysis. Emphasis is placed on the development in the rencent ten years since 1995.The other is research reports which is composed of 3 chapter as follows. Chapter 1 describes flow injection energy transfer chemiluminescence analysis of tobramycin based on N-bromosuccinimide (NBS)-fluorescein system. The method is based on the chemiluminescence emitted during the oxidation of tobramycin byN-bromosuccinimide (NBS) in alkaline medium in the presence of fluorescein as energy transfer. The detection limit was 0.070mg/L(3a), the linear range of determination was 0.20-100mg/L with the relative standard deviation of 2.1%(C=5.0mg/L ,n=11). The recommended method has been successfully used for analysis of commercial formulation and spiked plasma sample with satisfactory results.Chapter 2 describes flow injection chemiluminescence determination of amikacin. A new method for the determination of amikacin with flow injection chemiluminescence was described. It is based on the chemiluminescence emitted during the oxidation of amikacin by N-bromosuccinimide(NBS) in alkaline medium in the presence of fluorescein as energy transfer.This method is sensitive,simple,rapid and reproductively. Solutions can be analysed at a rate of 100 solutions h-1 with a RSD about 2.0% for determination of 5.0pg/mL of amikacin (n=11). The linear range of determination for amikacin was 0.50~70pg/mL. The detection limits is 0.080yg/mL(3o). The recommended method has been successfully used for analysis of commercial formulations without any sample pre-treatment.Chapter 3 describes flow injection chemiluminescence analysis of micronomicin. A new method for the determination of micronomicin with flow injection chemiluminescence was described. It is based on the chemiluminescence emitted during the oxidation of micronomicin by N-bromosuccinimide (NBS) in alkaline medium in the presence of fluorescein as energy transfer. This method is sensitive, simple, rapid and reproductively. The linear range of determination for micronomicin was 0.50-100ug/mL with the relative standard deviation of 1.8% (C=5.0]ug/mL,n=l 1). The detection limit was 0.080pg/mL(3a). The recommended method has been successfully used for analysis of commercial formulation with satisfactory results.