| Chemiluminescence (CL) analysis has received much attention for theanalysis of organic and inorganic species in a variety of fields for its high sensitivity,wide dynamic range and inexpensive instrumentation. Current research in CL analysis isfocused on two general directions. One direction is to discover new CL reactions andbroaden the analytical scope of CL analysis. The other direction is to combine theestablished CL systems with newly developing techniques, such as high performanceliquid chromatograph and capillary electrophoresis, to increase the applied value of CLmethod. The former is more important since it is the foundation of the CL analysis.Usually, CL is emitted during an oxidation-reduction reaction and many familiaroxidizing agents, such as potassium permanganate, cerium (Ⅳ), hypohalites and relatedoxidants, and so on, have been explored as the useful oxidant for liquid phase CLanalysis. Due to its hight reactivity, recently year, singlet oxygen has attracted muchattention in many fields. In many CL reactions, singlet oxygen has been frequentlysuggested as the reaction intermediate and played important role in these reactions.However, there have been few papers with the intention of using these species as theoxidant in the CL analysis. It's well known that singlet oxygen (1O2) can be produced inthe reaction between N-bromosuccinimide and hydrogen peroxide with high quantumyield. Based on this reaction, we explored the possibility of using singlet oxygen asoxidant in the CL analysis.This thesis consists of review and research section.In the first part, the electronic structure, properties and generate methods of singletoxygen was briefly introduced. Then the main detection methods and the utilization ofsinglet oxygen were summaried.The research section consists of four parts.Part one, we proposed a simple flow injection chemiluminescence (CL) method forthe determination of nitrofurazone. This method was was based on the reaction ofnitrofurazone with H2O2 and N-bromosuccinimide in alkaline condition. The CL signalwas proportional to the nitrofurazone concentration in the range 1.0×10-7～1.0×10-5g/mL. The detection limit was 2×10-8g/mL nitrofurazone and the relative standard deviation was less than 4% (6.0×10-6g/mL nitrofurazone, n=11). The proposedmethod was successfully applied to the determination of nitrofurazone in compoundfuracillin nasal drops, human plasma and urine samples. The CL reaction mechanismwas also discussed briefly.Part two, singlet oxygen generated in the reaction between N-bromosuccinimideand hydrogen peroxide was used for the chemiluminescence oxidation of imipramine.Strong chemiluminescence signal was observed when imipramine was mixed withN-bromosuccinimide and hydrogen peroxide in alkaline condition. The chemilumin-escence signal was linearly dependent on the concentration of imipramine in the rangeof 1.0×10-8～1.0×10-6g/mL. The detection limit was 5×10-9g/mL imipramine and therelative standard deviation was 1.5% for 4.0×10-7g/mL imipramine solution in elevenrepeated measurements. The proposed method was successfully applied to thedetermination of imipramine in tablets, in human plasma and in human urine.Part three, rutin was determined by a new flow injection chemiluminescencemethod, based upon its reaction with N-bromosuccinimide and hydrogen peroxide in thepresence of cetyltrimethylammonium bromide surfactant micelles. The chemilumines-cence signal was proportional to the concentration of turin in the range of 1.0×10-7～1.0×10-5g/mL with a detection limit of 7×10-8g/mL rutin. The relative standard deviationfor 1.0×10-6g/mL rutin solution was 2.2% (n=11). The proposed method wassuccessfully applied to the determination of rutin in four Chinese medicinal.Part four, the chemiluminescence reaction of phenol, N-bromosuccinimide andhydrogen peroxide in neutral aqueous medium in the presence of cetyltrimethy-lammonium bromide surfactant micelles was discovered. Based on this reaction, a newflow injection chemiluminescence system for the determination of phenol was proposed.The chemiluminescence signal was proportional to the concentration of phenol in therange of 1.0×10-7～1.0×10-5g/mL with a detection limit of 3×10-8g/mL rutin. The relativestandard deviation for 1.0×10-6g/mL phenol solution was 2.0% (n=11). The proposedmethod was successfully applied to the determination of phenol in phenol ear drops...
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