The Extraction, Isolation And Purification And Chemistry Structure Analysis Of Water Soluble Polysaccharides From Hemp Seed

In this paper, Hemp seed was defat alcohol,water-extracting,pepsin-enzymolysis, dialysing,freezing and drying.At last,the water soluble polysaccharides H was obtained.Il was fractionated by gel filtration chrom atograph on DEAE-sephadex A-50(Cl-) and Sepharose CL-2B and Sephadex G-100,the results finded two kinds of polysaccharides HS1 and HS2. The results showed that they were all homogeneous porysaccharides.Furthmore,molecular weight was determined by gel filtration chrom atograph method,the result was 42795 and 15396. The polysaccharides of HS1 and HS2 would degrade under the condition of high temperature,acidity (ph<7=and alkalinity (ph>) .The HS1 and HS2 were soluble in hot water but insoluble in the high concentration solutions of such organic solvents as alcohol,ether,acetone,acetic ether etc. By the gas chromatography analysis of polysaccharides HS1 was testified that the main ingredients of monosaccharide compsing the HS1 are D-Arabinose, D-Xylose, D-Mannose, D-Galactose and D-Glucose and the mole rate of them are 1.5: 1.2: 1.6: 2.0: 3.0.I.R.SPECTRA suggested that both HS1 and HS2 have p-glycoside linkage.