| Armillaria mellea is a kind of famous medicinal fungus symbiotic with Chinese medicinal herb "Gastrodia elata Blume" (Tianma). Tianma is regarded as one of the most important traditional Chinese herb. Many ancient pharmacists had considered that Tianma had an extensive treatment on many ailments. Recent studies showed that Tianma has the ability of enhancing the mentality, strengthening the brain and delaying the consenescence. Furthermore, it has some curative effect on senile dementia. Many studies and experiments showed that the mycelia and fermented liquid of Armillaria mellea have the similar pharmacological activity and clinically curative effect to Tianma. We have the ability in planting the "Tianma", but it is still suffered by the natural and the condition and the long growth period, so it is hard to satisfy the use of clinic. It is very important to replace Tianma by Armillaria mellea. Armillaria mellea contains abundant of components. Through the systemic study on Armillaria mellea, many chemical active compositions have been found, such as polysaccharide, sesquiterpenoids, purine and so on. The properties and bio-activities of those compounds were also studied. The functional experiments showed that Armillaria mellea had the ability to adjust the nerve system and have obvious sedation effect. It also has the ability of anti-convulsant effective and can raise the immunological competence of mice. Though there are large of compositions in Armillaria mellea, the function of each composition and the pharmic effect position of Armillaria mellea are not very clear. This give us a new look on exploiting more fungi resources and making full use of Armillaria mellea.The liquid submerged fermentation of mushroom is a new way of mushroom production. This method is of high yield, low cost, short fermentation time and simple technology comparing to that of solid fermentation. Plenty of mycelia and fermented liquid can be acquired through liquid submerged fermentation and effective compositions can be extracted from mycelia and fermented liquid.This paper as part of a project from Science and Technology Department of Jilin Province did some useful research. Below works were chiefly completed and correlated conclusions were obtained.1. The best Armillaria mellea strain was screened according to sleep experiments on mice combined with animal experiments.17 strains of Armillaria mellea were successfully isolated from Changbai Mountain. 8strains of Armillaria mellea were screened from 17 strains according to growing rate in solid media, sprouting time of mycelium and biomass in shake culture. In 8 strains, the best strain of sleep-regulated ability was screened according to sleep delitescence and sleep time experiments and comparison to the strain of Naoxinshu. An is regarded as the most ideal strain.2. The optimal culture media and culture conditions of liquid shaking flask were established.In order to screening the optimal culture media of Armillaria mellea, single factors tests in carbon and nitrogen sources were carried out. Through analysising the results of orthogonal experiment, the optimal culture media were screened out. Its compositions were as follows: potato 20%, glucose 1%, sucrose 1%, yeast extract 2%, silkworm powder 1%, KH2PO40.15%, MgSO4 0.075%, VB10.001%. And the optimal liquid shaking flask cultural conditions were as follows: initial culture media pH was 6.0; the cultural temperature was 271; the amount of each 500mL bottle was 200mL; the shaking speed was 160r/min; the cultural time of secondary liquid strain was 5 days; the addition of ethanol was 0.5%. And the highest mycelia biomass was 1.5250g/100mL.3. The optimal submerged fermentation cultural conditions were established.The optimal submerged fermentation cultural conditions were gained according to the orthogonal experiment. The conditions were as follows: the speed of stirring was 180r/min; the temperature was 271; the cultural time was 6 days. The mycelia biomass was 1.7050g/100mL.4. The fermentation dynamics curves...
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