Study Of The Fabrication Of Gold Nanotubule Array And Its Applications In Chemical And Biological Separations

Separation and detection of biological samples without any denaturalization has received much attention. Nanotubule technology, a newly developed but not unimportant method, highlights itself in its transporting and separating activities. It's been confirmed that solid supports are valuable replacements. Gold nanotubules, as a new kind of support, find their ways in separation tools and sensors with all the characteristics of single nanotubule. Together with other advantages, gold nanotubules, which hold great promise for bioseparation and bioanalysis, can be considered as an important tool of separation and detection of analytes.Gold was deposited on the surfaces and in the pores of the template polycarbonate membranes through a chemical method environment friendly. At room temperature, the gold nanotubule arrays are modified via self-assemble and imaged by atomic force microscope. Based on the fabrication, modification and imaging technology, a new method for the transporting and separating study of molecules is developed.Transporting of single molecules across the gold nanotubule arrays is investigated. The permeating activities of inorganic ions (Al3+ and Zn2+) are studied at different pH levels and explained by electric charge. Then the separation characteristics of tryptophan, morin, fluorescein and vitamin B2 by gold nanotubule modified with different function groups are observed. It is found that the separation of these substances through gold nanotubule is affected by their distinct structures. The transporting behavior of dichlorofluorescein, fluorescein sodium and fluorescein iso-thiocuanate with different substitutional groups is investigated. Then that of BS A and IgG through different modified gold nanotubule arrays at different pH levels is also discussed. The influence of pH levels on protein structure is analyzed using fluorescence anisotropism and a new principle is put forward.Separating of three pairs of moleculies (tryptophan/vitamin B2, hydrochloric aniline/Rhodamine B, and BSA/IgG) is progressed through modified gold nanotubulearrays. For the first pair, the relative separation results are 5.7 and 8.6 respectively and the absolute separation results are 111.5 and 168.0 respectively through cysteine and carbamidine thiocyante modified gold nanotubules. For the second pair, when pH level are1.98, 5.15 and 11.98, 1.8, 1.2 and 6.1 of the relative separation results are obtained. For the third pair, 31.6 and 23.1 are the relative separation results achieved when pH level is 4.5.