Preparation Of Gold And SiO 2 Nanocyclic Channels And Their Application In Chiral Separation

Nanochannel is a kind of the pore or tube with nanometer structure，havingdiameter of0.1to100nm. In the current research, nanochannel mainly involves thenatural protein ion channel and synthetic solid nanochannel. As an important branchof the nanobiotechnology，nanochannel technology has obtained widely attentions inrecent years. Because of the structure effect and physical chemical properties^nanochannel shows the unique advantages in gene sequencing, single molecularanalysis, the biomimetic ion channels design arid drug load.In this paper, the gold and silica nanochannels were prepared and characterized.The transportation of chiral substances through the functionalized nanochannelmembranes was investigated, and combined with surface enhanced Ramanspectroscopy on the online detection of the preliminary inquiry. Compared with thetraditional high performance liquid chromatography and capillary electrophoresis,there are few reports on the application of nanochannel technology to separate chiralsubstances. Details are presented in this thesis as follws:(1) Au was deposited within the pores of the polycarbonate template membraneusing an electroless deposition method. The process was greatly improved than thoseused before. Au nanochannels were characterized by SEM, TEM and AFM. The sizeof the pore can be precisely controlled by changing the deposition conditions.(2) L-cysteine was modified onto the gold nanochannels to render thenanotubules chiral and enantioselective behaviour ofr adsorption of chiral molecules.The migration of D-tryptophan and L-tyrosine through gold nanochannel and theeffects of pH and pore diameter of tubules on the separation for these two substanceswere discussed, and combined with surface enhanced Raman spectroscopy on theonline detection of the preliminary inquiry.. We can expect to provide sometheoretical support for more widely practical application on enantiomers separation ofnanaochannels. (3)ByUV spectroscopy study of p-cyclodextirn on the combined effect ofphenylalanine enantiomer package, discusses the inclusion mechanism, andinvestigated the effects of different experimental conditions on the inclusion action,providing the theory basis for the resolution of phenylalanine.(4)Anelectroless deposition method was used to deposit gold within the pores ofthe polycarbonate template membrane and gold nanochannels. then (3-cyclodextirnwas modified onto the gold nanotubulemembrane by gold sulfur bond，using p-cyclodextrin chiral selective recognition of DL phenylalanine to separate its. while thehydrogen bonding interaction in silica nanochannels membrane modifiedp-cyclodextrin can separate the DL phenylalanine, comparing the two kinds ofmodified membrane on repeated use of separation of enantiomers of phenylalanineeffect as well as the film, to seek more suitable resolution of chiral amino acids.