Nano Channels For The Flavonoids And The Biological Material Separation And Detection Research

As an important part of Nanoscience,nanochannels technique has become a new growthpoint.Nanochannel is the pores or tubles with diameter from 0.1 to 100 nanometers.It isexhibiting the great potential advantage and promising future due to the size effect andchemical physical characteristics.Nanochannel technique involves many disciplines such asmolecule biology,bio-chemistry,electronics,material science,informatics and so on.Littlepapers about nanochannel has been reported in our country.The extraction and separation of the antioxidant is a hot topic in food industry.Theantioxidant,a kind of food additive,can prevent the formation of corrupt toxic material,theoutflow of the nutrient content and the change of semblance luster of the food.Theantioxidant divides into many kinds from the chemical structure and origin,such as phenol,quinone type,amines,organic acid and salts,inorganic acid and salts etc.The flavanonebelongs to the kind of phenol antioxidant.There are many methods for the separation anddetection of flavanone,such as high performance liquid chromatography,gas chromatography,fluorescence method and so on.Many papers on the sepearation of small molecule based onthe Au nanotuble membrane has been reported.However,there are few reports on thedetection of the small molecule such as flavanone.Main work in the present thesis are shownas follows:(1)Au was deposited within the pores of the polycarbonate template memebrane usingan electroless deposition method.The size of the pore can be controlled by changing the dep-osition condition.SEM was used to characterize the pore size and the surface of membrane.The membranes are modified with some different chemistry group by self-assembly,which isused to separate and detecte the flavone and biologic substance.(2)The characteristics of rutin transportation through the nanotubule membranes wereinvestigated.The complex formed by rutin and Al³⁺was transported effectively through thegold nanotubule membrane modified with p-aminothiophenol under the existence of anelectric field.The method was verified by separation and determination of rutin from the rutintablets successfully with a recovery of 96.3%～99.3%. (3)The capture antibody,bovine IgG,was modified onto the inner wall of Aunanotubules by glutaraldehyde interlinking method.Goat anti-bovine IgG and goat anti-catIgG was separated by the gold nanotubule membrane with appropriate pore sizes based on theimmunoreaction between antibody and antigen with a coefficient of 5.6.In summary,we developed some new approach for bioanalysis based on nanochannels incombination with photochemistry and electrochemistry.These works will greatly benefit theresearch on separation and detection of flavone and biologic substance and developnanochannel techniques.