Study On Extraction, Separa-tion, Purification And Properties Of Lycopene

This paper mainly dealt with the extraction, separation and purification conditions of Lycopene and stability and function of the pigment. The study laid theoretical foundation for industrialization manufacture.Through experiments, the optimum extraction conditions of microwave extraction were obtained. The optimum conditions were that extraction temperature : 20 C; pH : 6 ; extraction solvent: Acetone ; the number of microwave power : 2 ; extraction time : 40s ; ratio of liquor and material=3:l ; the number of extraction : 3 . As a result, the yield of the extracted pigment achieved 95%.The optimum separation and purification conditions of Lycopene by column chromatogram were obtained. Absorbent: 100~200 silica gel; adsorption and desorption at normal temperature; ratio of Lycopene oleoresin and Acetone=l:10; desorption solvent : Acetone ; the flow rate on adsorption : 3.5mL/min ; HPLC conditions : chromatogrom column : C-18 ; flow phase: methanol: acetonitrile : dichloromethnae =45 : 10 : 45 ; the flow rate of transport solution : 0.6mL/min ; UV examiner ; wavelengh : 472nm . As a result, reappearition of experiment is good. The reproductive result of Lycopene : 90% ; 1.6% of Lycopene of tomato oleoresin were obtained .The stability of Lycopene had obviously enhanced after separation and purification. It is sensitive to light , heat , alkali , metal ion Fe3+ , Cu2+ , oxidants had destructive effect on Lycopene , reducing Agents had hardly effect . Antioxidion and preservative had protective effect. Lycopene had the effect of antioxidion and anticancer. The pigment showed preserve-stability and high - colored capacity in ice cream, beverage and cream.