Study On The Extraction Of Lycopene With Microwave And Enhancement Of Its Stability

It is found that lycopene has the function of anti-cancer, so nowadays study on lycopene is hot in science field. This paper mainly dealt with the extraction and purification of lycopene and its improvement of stability, The study laid theoretical foundation and reference for large-scale extraction and purification of lycopene.Through experiment, the optimum extraction conditions of solvent extraction and microwave extraction were obtained. The optimum conditions of solvent extraction were as follows: solvent was 6# solvent oil, the temperature of extraction was 35℃, twice extraction, ratio of tomato to solvent was 1 ' 2.5, the time of extraction was 3 hours, under these conditions, the yield of the pigment was up to 90%. But the optimum conditions of microwave extraction were as follows: solvent was 6* solvent oil, extraction power 800W,the time of extraction was 11 seconds, twice extraction, ratio of tomato to solvent was 1 : 2.5, As a result , the yield was beyond 90%. The color value of the extracted pigment was 11.6.Firstly the pigment that had been extracted was primarily purified: the fatty acid and fat that existed in the liquor of lycopene were turned into salt by the reaction of saponification, which could been dissolved by water, but lycopene was dissolved by organic solvent, so the fatty acid and fat were separated from the pigment. Through this process, the color value of lycopene was greatly increased, which was 29.8. Secondly in order to get rid of β-carotene which was the main impurity in the pigment, silica gel G was used in this experiment, the result of the optimum dry column filtration chromatography was reported in this thesis, liquor of elution was mixed solvents, including chloroform and oil aether, the ratio of them was 1:2.5. During this course, stepwise elution was adopted , as a whole ,the pigment were separated well and the elution curve was obtained, the color value of lycopene was 470. At last, the crystal of lycopene was gotten. Which was analyzed by UV spectrum and IR spectrum, the purity of lycopene was examined by HPLC.The stability of lycopene was reviewed, the thesis reported the effect of sun ,temperature ,acid, ion and food additive, the result showed that the pigment was sensitive to sun .temperature and oxidant ion.Much work had been done in order to enhance the stability of lycopene, as a result, by using the oxidant Vitamin E and β- cyclodextrin , the stability was extremely increased.