Study Of Ilex Latifolia Thunb. On Chemical Constituents And Biological Activities

Ilex.latifolia Thunb. grows widely in guangxi and guangdong province and has a broad resource. It is s kind of herbal medicine with wide treatment functions of the Yao nationality. We studtied the chemical constitunts of Ilex latifolia Thunb. from Liannan, and explored the antibacterial effective, relieving alcohol toxicity effect and liver protection effect ect.Five pure constituents and two mixtures were isolated from the Ilex.latifolia Thunb. Three pure constituents were determined as P -sitosterol( 1), urs!2 - ene-3 P , 28-diol(2), ursolic acid (3) by manifold spectroscopy methods such as IR, *HNMR, !3CNMR and MS ect., compound 4 and compound 5 are in determining. Two mixtures were identified as palmitic acid ethyl ester(6), palmitic acid n- butyl ester(7) and Ursa-9(1 l),12-dien-3- ol (8), B -Amyrin (9) by GC-MS.Compound 2 was isolated from Ilex.latifolia Thunb. firstly.The essential oil of Ilex latifolia Thunb.vsas extracted by steam distillation. The chemical constituents in the essential oil were analyzed by GC-MS. Forty-two components (acid,ester, alcohols, ketones, amides and some meterpenes)were identified. The contents of the constituents identified make up 83.46% of the total essential oil.To study the antibacterial activity of Ilex latifolia Thunb. in vitro by way of double-dilute method in plates of 96 wells. Three kinds of extractions of Ilex latifolia Thunb. (ethanol extg. ,n- butylol extg. and acetic acid ester extg. )all have antibacterial activities(MIC 2.50 mg/mL). The antibacterial activity of acetic acid ester extg. to Staphylococcus aureus and Escherichia coli was evident (MIC=0.625 mg/mL), the antibacterial activities of ethanol extg. and n- butylol extg. to Staphylococcus aureus were better(MIC=2.50mg/mL).To study the relieving alcohol toxicity effect and liver protection effect of Ilexlatifolia Thunb. and Ilex latifolia Thunb. -Flos Pueraria omeiensis (L P) with the alcohol toxicity model and the liver injure model made by CCl4 . The results showed Iand L & P can shorten sleeping time induced by alcohol, decrease alcohol concentration, reduce alcohol toxicity and protect the liver of mice injured by CCl4 and dec- rease the ALT activity. The low dose ,the middle dose of Ilex latifolia Thunb. and the highdose of L & P had better effect. In our experiment, we explored the effect to hypertension, CHL, TG level with rats. The results showed Ilex latifolia Thunb.can drop hypertension by drinking. Injecting Ilex latifolia Thunb. in vein had same effect and the effect has relation to the concentration of Ilex latifolia Thunb. The study showed that Ilex latifolia Thunb. can decrease the CHL and TG level in the serum of rats eating high fat foods , the decrement has relation to the concentr ation of Ilex latifolia Thunb. too.