Process Research Of Lycopene By Biosynthesis

Lycopene is an important carotenoid,and the ability to eliminate singlet oxygen quencher and free radical is much better than other carotenoids ；Because lycopene can induce information transfer of cells and reduce simultaneity cholesterin, so beneficial effect in the treatment and inhibitator of diseases such as cancer and atherosclerosis are behaved well and lycopene has received a great attention in recent years .The preparation of lycopene is very significant in the studies of lycopene. So far, there are 3 kinds methods of getting lycopene which are chemosynthesis ,extratration from plant by organic solvent and fermentation by microorganism. Compared with the other two method,fermentation has been widely studied for its lower cost.Now, Fermentation is one of potential methods for producing lycopene by Blakeslea trispora which is the highest yield in β-carotene industrially. In the paper, a new method in determination ,the choice of blocking agents, a systemic separation and purification and trisporic acid have been studied reasonedly.It is essential to establish an accurate and effective method on determining lycopene and β-carotene by HPLC to reduce the metrical error,because they are very instable when exposed to light and heat and very valuable. As a result,it is feasible to determine lycopene and β-carotene in our sample when sudan reagent is standard substance instead of lycopene andβ-carotene and the metrical error is lower than 5%. When they behave the following formula:Ylycopene peakaera =1.03Ysudan peakaera.In order to get lycopene from the fermentation ofβ-carotene, some bloking agents have been chosen. As a result, it is completely possible to achieve only lycopene without ?－carotene almostly in the presence of 2-amino-6-methylpyridine whose concentration is up to 1.0 g/L. When the concentration of tobacco dust employed in medium reaches 15g/L, The yield of lycopene is 58％ higher than the former ,which climbs to 1090.03mg/L , and there is little ?－carotene obtained simultaneity.In order to achieve an optimized route about separation and purification of lycopene by fermentation, the process about extraction , saponification and crystal have been established subsequently. Firstly,the optimal exatration condition has been rearched:the production reclaim is 93.5% when the proportion of extraction solvent Petroleum Ether and dry thalli is 20:1,the temperature is 70℃, the time is 20 min and extractive time is 3；secondly, we studied the different temperature ,time and the proportion of saponificated solvent and extraction.. It is indicated that we can achieved a best result that the proportion was 1:1, the temperature was 60℃ and the time was 30min. At the step,the percent of production losing is about 10；finally, incompact crystal instead of oiled crystal can be attained when the proportion of ethanol and concentrated saponificated solution is 5:1 under 4 ℃ for 12 hours,but about 39％ production has been lost at the process,so the condition of crystal must be optimized in the latter experiment . In addition to,pretreatment of wet thalli has been studied to remove fatty acid and else impurity in the process of lycopene extraction ,which can leave out saponification and get incompact crystal. the proportion of, New aspect of the method involves interaction at normal temperature,for 30 minutes and in the weight ratio ethanol and wet thalli is 85:15. The content of lycopene after pretreatment is improved 20%, and the dry period is storted to 12h . In the process of production, we found trisporic acid which is one of the outgrowths had played an important role in enhancing the yield of lycopene, so the preparation , extraction, stability trisporic acid have been studied in detail.