Isolation And Structure Elucidation Of Antitumor Compounds From Soil Actinomycetes

Microorganism is an important natural product resource. Its diversity and chemical complexity are treasury for development of new drug. Many researches found the microorganism metabolites had potential activities of antitumor, antivirus etc.Based on the forming machanism of tumor, selecting antitumor component from natural products by pharmacological screening is a fast. effective, advanced screening method. Meanwhile it is also one of the strategies of producing new antitumor drug.This paper established a screening method of soil actinomycetes metabolites for inhibition on K562 cell line but weak effects on normal Vero cell line by MTT assay in vitro.Of the 446 soil actinomycetes, 5 strains crude extracts have antitumor activities and 2278* was isolated further. Using ethyl acetate extraction, silica gel column chromatography and HPLC etc, a compound HYH-II-49I was isolated from its fermentation broth. The chemical structure of the active compound was tentatively elucided as an alkaloid by physico-chemical properties and spectral data of 1H-NMR, 13C-NMR and 2D-NMR respectively.