Synthesis Of The New Stationary Phase For High Performance Liquid Affinity Chro-matography With Dendritic Spacer Arm Using Poly (p-Phenyleneterephthalamide) (PPTA) As The Matrix

The purification and analysis of biomacromolecules are becoming more and more important as the fast development of biotechnology and bioengineering. In this work a new HPLAC stationary phase was synthesized, which could be used for biomacromolecular separation.The synthesis was processed in three steps. The first step was the synthesis of non-porous, 3-5 m PPTA microspheres, which were very suitable for using as the matrix of HPLAC stationary phase. The synthesis was taken place in the emulsified solution of water and tetrahydrofuran between p-phenylenediamine and terephthaloyl chloride, using emulsion polycondensation method. The reacting parameters, such as HLB value monomer concentration stirringvelocity and reacting time, were optimized in order to obtain the suitable microspheres. The second step was to add new type dendritic spacer arm to PPTA microspheres. Multipling the reactive sites on the surface of matrix was the main purpose of this step. The last step was the coupling of ligand-NAD to the spacer arm. The NAD was oxided by periodate first, then the active amino group on the surface of matrix couple to the periodate oxidized NAD directly to formate Schiff base, which could be then reduced by sodium borohydride and a new type [NAD-(PAMAM)-(PPTA)] stationary phase of HPLAC was finaly prepared.