Functional Siliceous Mesostructured Cellular Foam As Novel Stationary Phase And Drug Carrier

Siliceous mesostructured cellular foam?MCF?is a novel mesoporous silica material having attracted enormous attention for its potential application in adsorption,separation and drug delivery owing to its unique three-dimensional pore structure,large cage-like cell pores connected by windows,and well modifiable surfaces.This work aims to the preparation of novel functionalized mesostructured cellular foam silica for chromatography separation and drug delivery.The main contents are as follows:?1?Functionalized siliceous mesostructured cellular foam was prepared to achieve the mixed-mode and efficient separation of intact proteins.And molecular sieving effect for the first time played an important role in protein separation using mesoporous silica materials as HPLC stationary phase.The spherical silica particles were synthesized and the pore size was easily regulated by adding NH₄F as well as altering the aging time.After aminopropyl derivatization,the chromatographic performance of functionalized mesoporous silica particles was investigated in comparison with those without modification and commercial NH₂ column,and their mixed-mode retention mechanisms were investigated in detail.The superir separation performance for the retention of proteins was obtained on our home-made column in comparison with commercial NH₂column.Moreover,the home-made column showed similar performance for separation of polar anilines and neutral PAHs with the commercial column,owing to mixed-mode retention mechanisms including p-?stacking,hydrogen bonding,?-?EDA interaction and hydrophobic effect.All these results indicated that the aminopropyl modified MCF would be promising in the mixed-mode and efficient separation of biomolecules in addition with small molecules.?2?The synthesis,characterization,and application in biomolecular drug delivery was reported for monodisperse siliceous mesostructured cellular foam nanocomposites with mesoporous,magnetic,and luminescent properties.The composites exhibited tunable large pore size and monodisperse spherical morphology with narrow size distribution,which were favorable for biomolecular delivery.Additionally,they showed high magnetization and bright luminescence,which could favorably seek the diseased tissues and visualize the drug delivery system in real time when controllably releasing the drugs.The multifunctional nanocomposites were used as protein drug carrier to investigate the storage and release properties.The nanocomposites exhibited high protein loading amount.Drug release tests suggested that the composites have a controlled drug release property,which was strongly affected by the pH and ionic strength of the immobilizing solution.This nanocomposite could act as a multifunctional biomolecular drug carrier system,which could realize the targeting and monitoring of biomolecular drugs simultaneously.?3?Functionalized siliceous MCF coated magnetic oxide composites with large pore size,high surface area and strong magnetic response were obtained,and the pore size was affected by the way the magnetic oxide added.The nanocomposites showed fast adsorption for lysozyme with an adsorption capacity of 861 mg g^-1 in 30 min,and the pH of the immobilizing solution strongly affected lysozyme release trends.We proposed a novel approach to adjust the morphology and pore structure of core-shell meterials.It has been shown that functionalized siliceous MCF are potential materials in the adsorption and delivery of proteins.