Application Of Chromatography Dynamics To The Separation Of Traditional Chinese Medicine

Chromatography has been gradually used as a purification method in the Traditional Chinese Medicine (TCM) industries and research recently. In this study, mathematic models of chromatographic process for the purification of TCM were built and applied to optimize operation parameters and scale up.First , an equilibrium-dispersive (ED) model was used to describe the chromatographic separation of Naringin and Neohesperidin. In this model, a competitive isotherm was adopted and determined by the inverse method developed by G. Guiochon and his coworker. The excellent agreement between the model predictions and the experiment results was observed.Then, taken the yield as the objective function, the built ED model was used to determine the optimum separation conditions of Naringin and Neohesperidin. The sample size and the flow rate were optimized to achieve maximum production rate of Neohesperidin. The recover yield, purity and productivity of Neohesperidin at different sample size and flow rate were also investigated, respectively.Furthermore, a general rate (GR) model including axial dispersion, interfacial film mass transfer and intraparticle diffusion was built to describe the chromatographic separation of puerarin and daidzein on a macroporous resin column. The accuracy of this model was verified by comparing its predictions with the experimental data. The effects of flow rate of elution to the isolation of puerarin and daidzein was also investigated. The simulation result shows that this mathematic model is a useful tool for optimization of operating parameters.