The Study Of Drug-Protein Interaction In Vitro Based On Fluorescence Detection

When a drug enters into the interior of the body, it often undergoes the processes of absortion, distribution and excretion. A large number of drugs would generate a kind or several kinds of new compounds—metabolite, passing through the reaction of metabolism. The drug and the metabolite can also bond with biomacromolecule, such as receptor, tissue and plasma protein, in different degree. In the process of transportation and translation, drug would bond with tissue protein, including receptor, and body fluid protein. Therefore, beside the unbound drug and metabolite, there are still bound drug and metabolite in tissue and body fluid. Passing through body fluid, drug was transferred to each organ and tissue. Free drug in the plasma transfers freely to the target organ, whereas bound drug hardly passes through the blood capillary walls to reach the action site. In this respect, plasma protein binding is an important factor in establishing pharmacokinitic and pharmacodynamic properties of a drug, as only the tree fraction of the drug is pharmacologically active. Some important pharmacokinitic properties such as hepatic metabolism rate, renal excretion rate, biomembrane permeation rate, and the steady state distribution volume, also depend on the unbound drug fraction. Thus, the study of drug-protein binding in body fluid is of great importance.The thesis consists of two parts. The first is a review dealing with the application of fluorescence in pharmaceutical. Emphasis is placed on the development in recent ten years.The other is reseach reports which is composed of 2 chapters.Chapter 1 describes the study of drug-protein interaction in vitro based on fluorescence detection, and three sections are contained in this chapter. The first...