| Dracocephalum moldavica L. (DML), as a Uighur folk medicinal herb, is a plant belonging to the Labiatae family and is known and used by Uighur people since antiquity. For extensive pharmacological effects, DML has been increasingly paid more attention by researchers. Recently, researches on DML are centered on clinical therapy coronary heart disease, atherosclerosis, hyperpiesia, myocardial ischemia, but few researches were investigated about chemical components. Furthermore, no study on the leaching characteristics and speciation of trace metal elements and the antioxidative activities research of effective chemical components in DML has ever been reported. In this paper, for exploring and clarifying DML's pharmacological mechanism, physical and chemical methods were adopted to study leaching characteristics, speciation, and distribution of decoction in stomach, intestine of trace metal elements in DML and effective chemical components'antioxidative activities and immunomodulatary activities on molecule level. In chapter one, the advances of trace metal elements, antioxidative activities and DML's studying were reviewed. In chapter two, the method of secondary level of the speciation analysis and the method of n-C8H17OH—H2O system simulating the distribution in the body's stomach and intestine of six trace elements in DML, were proposed by Flame Atomic Absorption Spectrophotometry (FAAS). Meanwhile, octanol-and water-solubility concentration of six trace elements under gastric and intestinal acidity were determined, analyzed and compared by FAAS. Results showed that six trace elements in DML existed in mixed forms but existed mainly in one form, respectively; the different acidities of digestive site had affected greatly the existing species of trace elements; Zn/Cu value was 1.11 in decoction, which made contribution to improving Zn/Cu value in serum of coronary heart disease patient. In chapter three, Dracocephalum Moldavica Polysaccharides (DMP) were obtained by hot water extraction, ethanol precipitation, Sevag method deproteinization and subsequently purified by different organic solvent. Polysaccharides content was determined by phenol-vitriolic colorimetry. Meanwhile, the scavenging effects of DMP on active oxygen free radical were measured with Vc and Lycium barbarum Polysaccharides as control sample. The results showed that the polysaccharides content of DMP was 6.38%,the method was of high accuracy and strong scavenging effects of DMP on O2ˉ·,·OH, H2O2 and reducing power of DMP. Moreover, their activities increased with concentration of polysaccharides increasing and were stronger than Lycium barbarum Polysaccharides. In chapter four, different extracts in DML extracted were water fraction (WF), ethanol fraction (EF), petroleum ether fraction (PEF), trichloromethane fraction (TCF), ethyl acetate fration (EAF) and n-butanol fraction (NBF). The antioxidant abilities of different extracts in DML were evaluated by scavenging active free radical and reducing power, which enable to find the strongest activity fraction in DML. The results indicated that most of extracts were of better active free radical scavenging activities; EAF showed the strongest antioxidant activity among extracts; EF's scavenging activity was stronger than WF's. In chapter five, Dracocephalum Moldavica fatty acid extracted with Soxhlet extractor by organic solvents was saponified and methylated, and then the products were qualitatively and quantitatively analyzed by GC-MS with satisfactory results. Seven fatty acids were identified. Of which unsaturated acids account for 70.89%,...
|
PDF Full Text Request