Study On The Chemiluminescence Behavior Of Some Antibiotic And Hormone And Their Applications To Analysis

A analysis method that based on chemiluminescence (CL) reactions is sensitive, fast> simple and easy to control and has its special advantage in environmental analysis, pharmaceutical analysis and medical study.The thesis consists of two parts.In first part (Chapter one), the general introduction, the method of CL, the applications of CL methods in environmental and pharmaceutical analysis and the thought and content of this thesis were described.The second part (Chapter two to Chapter four) consists of research report.In Chapter two, studies on some new flow-injection CL methods for the continuous determination of fluoquinolone (norfloxacin) were as follows:1) Sodium dodecylsulfate can greatly enhance the emission intensity of the weak chemiluminescence (CL) reaction of Ce(IV) and norfloxacin in acid medium. Based on the phenomenon, a simply and rapidly new method for the continuous determination of norfloxacin is developed. The calibration graph is linear for 7.78xl0~-8~5.82xl0~-6g/mL, the detection limit(S/N=3) is 1.57x10~-8g/mL The method was applied successfully to the determination of norfloxacin in capsules. Additionally, the system's mechanism was discussed.2) Base on norfloxacin can sensitize the chemiluminescence system of potassium permanganate-sodium thiosulfate in sulfuric acid medium, a new flow injection chemiluminescence method has been studied. The calibration graph is linear for 2.00xl0~-8~2.00x10~-6g/mL, the detection limit(S/N=3) is 8.36x10~-9g/mL. The method was applied successfully to the determination of norfloxacin in capsules. Additionally, the system's mechanism was discussed.3) The method is based on the CL reaction of norfloxacin with sodium thiosulfate and Ce(IV) in sulfuric acid medium sensitized by sodium dodecylsulfate. Based on the phenomenon, a fast, simple and sensitive new method for the continuous determination of norfloxacin is developed. Under the optimum conditions,the CL intensity is proportional to the concentration of the norfloxacin in the range of 3.89xl0'8~7.18xl0'6g/mL. The detection ijmits (S/N=3) were 2.21xlO-9g/mL fornorfloxacin. The method has been applied successfully to the determination of norfloxacin in pharmaceutical formulation and human urine. The mechanism for this chemiluminescent system was also discussed.In Chapter three, a novel flow injection chemiluminescence (CL) method for the fast, simple and sensitive determination of three estrogens, namely dienestrol, diethylstilbestrol and hexestrol, has been investigated. It is based upon the CL reaction of the studied estrogens with formaldehyde and potassium permanganate in sulfuric acid medium. Under the optimum conditions, the linear ranges for the determination of estrogens were 8.00x10"9~8.00xl0"6g/mL for dienestrol, 5.00xl0'9~4.75xl0'6g/mL for diethylstilbestrol, and 2.00x10"8~5.00xl0'6g/niL for hexestrol. The detection limits (S/N=3) were 2.61 xlO"9 g/mL for dienestrol, 1.08xl0"9 g/mL for diethylstilbestrol and 6.28x10'9 g/mL for hexestrol. The proposed method has been successfully applied to the determination of diethylstilbestrol in tablets and three estrogens in tap water. Furthermore, the possible reaction mechanism was also discussed in this paper.In Chapter four, the ability of carbendazim to enhance the CL reaction of luminol-H2O2 in an alkaline medium has been exploited to develop a novel flow injection CL method for the determination of carbendazim. Under the optimum conditions, the linearity of the calibration graphs for the determination of carbendazim is in the range 2.00x10"8~2.00xl0'6g/mL with a detection limit (S/N=3) of 7.24xlO"9 g/mL. The proposed method has been applied to the determination of carbendazim in tap water samples. Furthermore, the possible reaction mechanism was also discussed in this paper.