Determination Of Organic Pollutants In Water With Flow-injection Chemiluminescence Detection

Flow-injection chemiluminescence analysis （FI-CL） which is the combination of FIA with CLdetection is a high sensitive trace method. It detects the concentration of the analyte according to the CLintensity at some points of certain chemiluminescence reaction.This analysis technique has so manyadvantages. such as high sensitivity, rapidity and reproducibility in signal detection, convenient apparatusand operation, easy to realize automatic and continuous analysis etc.nowadays, FI-CL has been applied toareas of environmental detect, biomedical, medicament analysis, food analysis, immunoassay, analysis ofdustry and agriculture online and so on..This thesis realizes some environmental samples of the organic pollutants containing the measurementmethod of the chemical luminescence and discuss the mechanism research，which mainly using flowinjection chemiluminescence analysis method. The paper includes two parts:1. It depicts the basic principle, advantages, application, development history and trend ofdevelopment of CL; It introduces experiment devices of the FIA and FI-CL analysis technology and severalclassic chemiluminescence reagents; The paper summarizes some applications of the FI-CL analysistechnology in the fied of environmental analysis.2. In this paper, we mainly studied the FI-CL analysis method for the determination of trace Catechol，P-aminoazobenzene and Bisphenol A in environmental water samples.（1） Determination of Catechol in environmental water samples with flow-Injectionchemiluminescence analysis.It was found that Ag nanoparticles （NPs） could enhance the chemiluminescence （CL） intensity ofluminol-potassium ferricyanide system. On the basis of enhancement effect, a flow injection method wasdeveloped for the determination of catechol.In the optimum conditions, the linear range was5.0×10^-5～1.0×10^-2mg/L and the detection limit was4.8×10^-5mg/L. The relative standard deviation （RSD）for8.0×10^-4mg/L Catechol （n=11） was2.3%. The method was used to detect the concentration of catecholin water samples with satisfactory result.（2） Determination of trace p-aminoazobenzene in environmental water using flow-injection chemiluminescence analysis.In the acidic medium, p-aminoazobenzene can enhance the intensity of Chemiluminescence ofKMnO₄-HCHO system. Accordingly, a new method was established for the first time to detectp-aminoazobenzene with flow-injection chemiluminescence techicque. In the optimum conditions, thelinear range was2.0×10^-7～2.0×10^-5g/mL and the detection limit was9.4×10-8g/mL. The relative standarddeviation （RSD） was2.1%. The method was used to detect the concentration of p-aminoazobenzene inwater samples with satisfactory result.（3） Determination of trace Bisphenol A in environmental water samples using flow-injectionchemiluminescence analysisBased on the inhibition effect of Bisphenol A on the chemiluminescence generated from theLuminal-H₂O₂reaction in alkaline Bisphenol A, Under optimized conditions, the decrease ofchemiluminescence intensity was linear with Bisphenol A concentration in the range of0.5¹0μg/mL, andthe limit of detection is0.23μg/mL, The relative standard deviation（n=11）is2.6%for2.0μg/mLBisphenol A, The method was applied to the determination of Bisphenol A in water samples withsatisfactory results.