| In this paper, normal nutrients (include sugars, vitamins, amino acids, mineralsubstances, etc.) in Guyuelongshan Shaoxing rice wine were firstly quantificationallydetermined. And secondly using modern analytical and measuring methods systematicallyand deep studied some biofunctional constituents in rice wine at the first time in our country,which contain phenols, functional oligosaccharides, GABA, functional peptides, etc.1,Use acetic ester stepwise extraction in acid and neutral conditions to separate acidicand neutral phenols. After these phenols were subjected to RP-HPLC and gradient eluted ,the contents of eleven phenols (catechin, epicatechin,rutin, chlorogenic acid,,ferulic acid,etc.) in rice wines of different years and different processes were determined.2,Firstly by using volume exclusion chromatography and cation exchange column,sugar molecular-weight distribution and the contents of monosaccharide, disaccharide andtrisaccharide in rice wine were determined. And then by using amino-bonding phasecolumn,the contents of three functional oligosaccharide (isomaltose, panose, isomaltotriose)in shaoxing rice wine of different years and different processes were determined andstudied.3,Using OPA-FMOC pre-column derivatization HPLC first determined there are richphysiologic functional contents—inhibitive neurotransmitter—GABA in GuyuelongshanShaoxing rice wine sample .4,Peptides in rice wine were isolated and purified by ultrafiltration followed bymacroporous absorption resin chromatography, gel filtration chromatography and RP-HPLC . And the peptides present hypotensive and cholesterol-lowering activity in vitro. Thesequence of four hypotensive peptides and one cholesterol-lowering peptide wereGln-Ser-Gly-Pro, Val-Glu-Asp-Gly-Gly-Val, Pro-Ser-Thr, Asn-Thr, and Cys-Gly-Gly-Ser,which were analyzed by LC/ESI-MS and LC/MALDI-TOF-TOF-MS. For the first time therelationship between structure and activity of the functional peptides in rice wine werestudied on molecular level.
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