Application Of Inductively Coupled Plasma Mass Spectrometry In Speciation And Biological Analysis

In this paper the application of Inductively Coupled Plasma Mass Spectrometry in speciation and biological analysis are studied. In speciation analysis, a method to determine trace Sb(Ⅲ) and Sb(Ⅴ) in environment by ion chromatography coupled with inductively coupled plasma mass spectrometric detection was developed. Sb(Ⅲ) and Sb( Ⅴ) were separated on a DIONEX IonPacAS14 (4.6 mm×250 mm id) column with 3mg/mL EDTA and 0.2mg/mL potassium hydrogen-phthalate (KHP) (pH 4.7) eluent solution at 1 mL/min. The detection limit for was Sb(Ⅲ) 1.62μg/L, and 0.36μg/L for Sb( Ⅴ). The linear range was from 2.0(μg/L to 200μg/L. With regard to reproducibility, R.S.D (n=6) was 2.15% for Sb(Ⅲ) and 3.24% for Sb(Ⅴ). The methods were applied to determine of Sb(Ⅲ) and Sb(Ⅴ) in water samples. In biological analysis, a sensitive method for the determination of Bi by inductively coupled plasma mass spectrometry in the blood plasma of those who take the bismuth medicament for the treatment of gastric ulcer is described. The instrument parameters for determination of Bi have been optimized and the method have been evaluated. The linearity range of the standard curves is 0.05—10.0 Mg/L. The RSD of the method, evaluated by replicate analyses of quality-control samples whose bismuth concentrations are 0.30, 8.0 and 80.0 Mg/L, are 1.2—11.0 %, 1.6—5.6 %, 1.6—5.2 % ( 1 day, 5 times ,n=6) and 6.5 % (0.30 Mg/L), 2.9% (8.0 Mg/L), 0.7% (80.0 Mg/L) ( 5 days, n=6), respectively. The relative average recoveries are 103 %(0.30 Mg/L), 98 %(8.0 Mg/L), 99 %(80.0 Mg/L) separately. In a word, this method is simple, convenient and feasible. Its satisfying results does provide scientific gist for the pharmacokinetics study of bismuth compounds.