Synthesis And Property Research Of Magnetic Polymer Microspheres

The magnetic microspheres usually consist of a magnetic core and polymer shell. They can be rapidly separated from the mixtures and have the characters of the general microshpheres, so they be attended by more and more people. The magnetic polymer microspheres can be applied in various aspects in biotechnology and biomedical engineering, such as cell separation, immobilized enzyme, protein separations, target drug,and biochemical assays, etc. In this study, the magnetic nanoparticles have been prepared in a modified co-precipitation procedure at first. Then the magnetic polymer-coated microspheres were prepared by the microemulsion polymerization based on the magnetic nanoparticles. The size, dispersion and morphological characterizations of the magnetic microspheres were carried out with an optical microscopy, SEM and TEM. The magnetism can be observed clearly using a magnet of magnetic field about 3000 GS. The magnetic properties of the microspheres were evaluated using a vibrating sample magnetometer (VSM). The degree of the thermal stability of the magnetic microspheres was carried out with a thermogravimetric analysis. The functional groups on the surface were carried out with Furier transfer infrared (FTIR). The fluorescence microscope was used to investigate the avidin linked microspheres. The reagent kit HCG SEROZYME was used to check the microspheres'capacity of conjugation with FITC. The results indicated that the magnetic microspheres were superparamagnetic, well shaped spheres, mono-dispersed with abundant functional groups on the surfaces of the magnetic microspheres and good thermal stability. The microshpheres could be linked well with the avidin and FITC antibody.