| In this article, Taking Escherichia coli, Staphylococcus aureus and Bacillussubtilis as target bacteria, the germicidal and inhibitory efficiency ofnano-antimicrobial is tested through suspension quantitative bactericidal test andinhibitory test, and the performance of anti-bacteria of nano-thin films is also studied.The MIC and MBC of nanosized TiO2 and nanosized ZnO are determined in the firststep of the research, which provide a reference to the practical use ofnano-antimicrobial. Then the factors influencing the performance of anti-bacteria ofantimicrobial are studied through experiment, and the factors include antimicrobialconcentration, illumination time, pH and different crystalloid of antimicrobial.Furthermore, the performance of anti-bacteria of nano-antimicrobial exhibits directlythorough the test of inhibition ring. In the anti-bacteria test of the nano-thin films, thedifference of the performance of anti-bacteria of nanometer materials that by differentheat treatments is compared, the effect of dipping in different media on theperformance of antimicrobial is discussed, and the influence on the performance ofantimicrobial by diverse function time of antimicrobial is also tested.The testing results show that the inhibitory efficiency of the experimental objectthat MBC is in the range of 1×107～2×107cfu/mL can reach the high rate of 99.99% when MIC of nano-antimicrobial is above 1000ppm. In the anti-bacteria test of thenano-thin films, the anti-bacteria effect comes to be best when the temperature ofheat treatment is 400℃, and the inhibitory efficiency decrease as the temperature ofheat treatment goes up. The nano-thin films is studied by XRD and SEM, and theresults show that the surface of the nano-thin films is smooth and uniform when thetemperature of heat treatment reach 400℃. Moreover, the thickness of the films isbetween 300 and 400 nanometer and is less than the wave length of the visible light,which makes the films show good performance of permeating light.In addition, the anti-bacterial effect of antimicrobial shows nearly no change asthe function time increase from 1d to 7d, which demonstrate the good anti-bacteriadurability of nano-antimicrobial. Moreover, the antimicrobial all shows anti-bacteriaeffect to different bacteria, which prove the nano-antimicrobial have a goodanti-bacteria spectrum. |
