Study On Preparation And Antimicrobial Activity Of P(HEA-co-AA)/Ag Nanocomposite Sol

The copolymer P?HEA-co-AA?was synthesized by AA and HEA with the method of free-radical solution polymerization.The colloids of P?HEA-co-AA?/Ag was prepared from liquid phase reduction method with AgNO₃ as precursor and P?HEA-co-AA?as the reducing agent and stabilizing agent at the same time.The silver nanoparticle size of 20²00 nm was controlled by adjusting neutralization degree of the copolymer,reaction temperature,composition and content of P?HEA-co-AA?,dosage of AgNO₃.The morphological structure and chemical composition of P?HEA-co-AA?/Ag colloids were characterized by Transmission Electron Microscope?TEM?,Laser Particle Size Distribution,Fourier Transform Infrared Spectrometer?FTIR?,Thermal Gravimetric Analysis-Differential Scanning Calorimeter?TGA-DSC?and so on.The results showed that the silver nanoparticle is ellipsoid,uniform particle size distribution of narrow particle size,silver nanoparticle dispersed in polymer equably and polymer play a role of dispersing agent and reducing agent.Test on the Gram-negative bacteria?Escherichia coli?and Gram-positive bacteria?Staphylococcus aureus?by applying P?HEA-co-AA?/Ag colloids as the antimicrobial.With the antimicrobial to two types of bacteriums do inhibition zone.The experiment results show that,in a certain range?80¹18 nm?of silver nanoparticles,the bacteriostasis is better when the particle diameter smaller;However,when the silver nanoparticle size is less than 80 nm,the bacteriostasis remain unchanged with the variation of the silver nano particle size.The analysis of the MIC and MBC by applying the antimicrobial with distinctive silver nanoparticle sizes indicates that when the silver nanoparticle size is greater than 80 nm,the MIC and MBC for the two kinds of bacteria will decrease with the reduction of the silver nanoparticle size;while when the silver nanoparticle size is smaller than 80 nm,the MIC and MBC for the two types of bacteria generally remain unchanged with the variation of the silver nanoparticle size.The P?HEA-co-AA?/Ag colloids has outstanding antibacterial function toward both the gram-negative bacterium and gram-positive bacterium,with slightly better antibacterial activity toward gram-negative bacterium than gram-positive bacterium.The P?HEA-co-AA?/Ag colloids has a broad prospect of application from many aspects,such as the simple methods for preparation,the low costs and the excellent antibacterial properties.