The Study On Extract And Purification Of Active Componen Of Momordica Charantia And Biology Active

Momordica Charantia has the medical and edible value. In India, momordica charantia was used to cure the sugar diabetes. At present, people more and more regard the characteristic of falling blood sugar. Aim at the applied foreground of the momordicosides and momordica polysaccharide, this paper colligated the existing extract crafhvork of the momordicosides and polysaccharide and research method in recent years, expatiated the mensuration of the momordicosides and polysaccharide, and confirmed the condition of extract, membrane separation and crystal. At last, we researched the biology active of the momordicosides.1. We can protract the standard curve by spectrophotometer, in range of this concentration, we can use the regress equation and account the content of active substance. The regress equation of momordica polysaccharide is: y=0.0041x-0.0057, R~2=0.9997;The regress equation of ginseng sponin is: y=0.1647x-0.0026 , R~2=0.9988;The regress equation of self-restrain momordicosides is: y=lE-0.4x-0.0005, R~2=0.9977;The regress equation of reducing sugar is: y=0.0528x+0.0236, R~2=0.99742. By comparing the water-extract and alcohol-extract and doing the orthogonal experiment, we can confirm the best craftwork condition of water-extract: 70℃, 3h, the rate of materiel and water is 1:20. If we repeat 3 times, its extract rate can extend 85%.3. The best craftwork condition of membrane separation: the pressure is 0.02Mpa, the temperature is room temperature.4. Experiment indicate: AB-8 type macro pore adsorption colophony is suit to adsorb momordica polysaccharide, the best craftwork condition :the flow of adsorption is 2ml/min, the flow of desorption is 14ml/min, room temperature, normal pressure, the capability of absorption is 150ml/500cm~2, the absorption rateis 58.32%;NKA type macro pore adsorption colophony is suit to adsorb momordicosides, the best craftwork condition :the flow of adsorption is 2ml/min, the flow of desorption is 14ml/min, room temperature, normal pressure, the capability of absorption is 200ml/500cm2, the absorption rate is 69.73%;the method of momordicosides crystal is a ether deposition;the method of momordica polysaccharide crystal is freezing desiccation.5. Short of the standard momordicosides, by the HPLC the crystal that we extracted ourselves is consistent with the momordica juice, its concentration is 94.42%. Subsequently, we can confirm its structure by MR.6. Animal experiment indicate: momordicosides has the remarkable effect of falling blood sugar and it can cure diabetes by many approach. So, momordicosides has positive meaning for curing the diabetes.