Studies On The Anti-HIV Active Ingredient Of Tea & Antibacterial Activity Of Momordica Grosvenori Leaf And Stem

This project divides into following two parts1. Studies on the Anti-HIV Active Ingredient of TeaIn order to seek and discover anti-HIV bioactivated natural products from Chinese herbal medicine, activity screening was performed in crude extracts from over 100 kinds of Chinese herbal medicine. The model is about the HIV-1 LTR in macrophage cell line BF24. HIV-1 LTR will be activated under Porphyromonas gingivalis stimulation and the reporter gene (luciferase) will be expressed. The luciferase activity will be measured by CAT-ELISA. It was found that the 75% ethanol extract of tea showed the effect of down regulation of LTR and confirmed the fraction ethyl acetate was the most effective fraction. In this project, isolation and purfication of the fraction ethyl acetate was performed guiding by anti-HIV activities. Five fractions(petroleum ether/acetic ether=5:1, 3:1, 1:2, 1:3, 1:5)which had the remarkable activities were isolated from the fraction acetic ether. We got 5 compounds from these fractions. Two of them were identified as caffeine andβ-sitosterol.2.Preliminary Research on antibacterial activity of the Ethanol extracts from Momordica Grosvenori leaf and stemMomordica grosvenori belongs to a Siraitia grosvenon plant. It had the functions of moisturizing lung、dispelling Phlegm and relieving cough virtues. So it is used widely in manufacturing medicine, beverage and flavoring. Predecessors were mainly focused on Momordica grosvenori fruits and Momordica grosvenori while there were few studies on the leaf and stem.This part dicussed antibacterial activity of the ethanol extracts from Momordica grosvenori leaf and stem on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Micrococcus luteus and Candida albicansil. The bacteriostatic activity of the ethanol extracts of leaf and stem on Pseudomonas aeruginosa is the highest, and the bacteriostatic rate were 90.9% and 76.7% when the concentration was 50mg/mL. The bacteriostatic activity of the ethanol extracts of stem on E. coli is better while the bacteriostatic rate was 76.3% when the concentration was 50mg/mL.