Study On Coimmobilization Of Lignin Peroxidase And Glyoxal Oxidase And Their Application In Asymmetric Synthesis

In this thesis, lignin peroxidase(LiP) ;and glyoxal oxidase(GLOX) production were first produced by the white-rot fungus Phanerochaete chrysosporium SHL-1 in N-limiting cultures. Secondly, the two enzymes were purified rudely. Thirdly, they were coimmobilized on Chitosan. Finally, the two enzymes were applied in asymmetric synthesis.During production of LiP and GLOX by Phanerochaete chrysosporium SHL-1 in N-limiting cultures, the optimal carbon source and nitrogen source were glucose and ammonium tartrate; the optimal concentration of carbon was 15 g/L and 10 g/L respectively for Lip and GLOX; the concentration of optimal nitrogen was 0.2 g/L and 0.3 g/L respectively; After two days, the optimal volume decanted was 50%; the optimal volume was 50ml/250ml flask; the optimal frequency of flushing pure O₂ was once a day; the optimal rotating speed was 80rpm; the optimal seed amount was 3×10⁷/flask; the optimal temperature was 37℃; the...