Study On Biodegradation Of Compound Bacteria On Oil Of Shengli Oil Field

In the process of crude oil extraction and refining, large quantities of petroleum pollutants were produced, it is estimated that 8 million tons petroleum was poured into underground water, ground water and soil through various approach in one year in the world. Petroleum pollution brings a great danger to ocean fishery resource, destroy the natural structure of soil and affect the ecological balance.Bioremediation of petroleum pollutants has performed a great promise as a potentially effective and low-cost treatment option. However, the low bioavailability of hydrocarbons leads to their slow biodegradation. An option to enhance the bioavailability of crude oil is to add bacteria who can produce biosurfactants to emulsify crude oil. with its broad-spectrum degradation ability, white rot fungi has a excellent degradation of crude oil.In this study, 3 strains capable of producing biosurfactants were isolated from well sit waste water at Shengli and grow up. These biosurfactant-producing microorganisms were investigated for their potential in enhancing bioavailability and biodegradation of crude oil and their impacts on the capability of white rot fungi in crude oil biodegradation. The main results were summarized as follows:Respectively, the three strains had especially excellent surface ability. The biosurfactants which were produced by zsh7 and zshlO were lipidpeptide and the biosurfactants produced by zsh11 was glycolipid type.The results showed that the three bacterial strains could make the diameter of oil membrane spot spread more than 19cm. Crude oil was degraded well by inoculated white rot fungi after being pretreated by each isolated bacterial strain, the degradation ratios could be enhanced from 71% to 90.4%, 91 % , 92% in seven days.