Font Size: a A A

Influence Of Heavy Metal Cobalt Ion On The Electron Transfer Activity And Structure Of Thylakoid Membrane

Posted on:2007-03-30Degree:MasterType:Thesis
Country:ChinaCandidate:Y W ChenFull Text:PDF
GTID:2121360185494196Subject:Biochemistry and Molecular Biology
Abstract/Summary:PDF Full Text Request
The photosynthesis is the most important chemical reaction on our earth. The thylakoid membrane plays a very important role in photosynthesis. The Photosystem II (PSII) is one of the most important thylakoid-bond protein-pigment complexes. It is very significant for us to do research on PSII in order to know how to raise the efficiency of transforming light to energy and of simulating the sun energy in the future. It is well known that Co2+ is a graveness factor to the environment pollution. We purified the thylakoid membrane and PSII particles from Ophiopogon tuber L. and Spinacia oleracea L.. We used the methods of absorption spectra, fluorescence emission spectra, activity of DCIP photoreduction, SDS-PAGE and native PAGE to investigate effects of Co2+ ion on thylakoid membrane and PSII particles. The results suggested that Co2+ ion can act on releasing oxygen side of PSII particles.Firstly, effects of Co2+ on fluoresence emission spectra at room temperature, activity of DCIP photoreduction and polypeptide compositions of thylakoid membrane from Ophiopogon tuber L. were studied. The results showed that fluoresence emission spectra at room temperature was changed after Co2+ treatment: intrinsic fluoresence spectra at room temperature upon excitation at 278 nm and 295 nm was decreased at the Co2+ concentration below 15 mmol/L and increased at the Co2+ concentration above 15 mmol/L; Chlorophyll fluoresence spectra at room temperature upon excitation at 436 nm and 475 nm was decreased; The inhibition of DCIP photoreduction activity and the changes of polypeptide compositons of the...
Keywords/Search Tags:Thylakoid, Photosystem II, Electron transfer activity, Absorption spectrum, Fluorescence emission spectrum
PDF Full Text Request
Related items