Preliminary Study On Extraction Of Dihydromyricetin From Ampelopsis With Supercritical CO₂ Fluid Technology And The Inhibition Experiment

This topic uses the Supercritical CO₂ Fluid Technology to extract dihydromyricetin from the Ampelopsis grossedentata. Some factors influencing the dihydromyricetin extraction from the Ampelopsis grossedentata were studied under the supercriticality, including the influence of extracting pressure, temperature, time, and CO₂ flow rate. Based on the 1L supercriticality-extracting experiment, further uses the 5L supercriticality-extracting equipment to carry on the enlarged experiment. The optimum conditions for the extraction were shown as following: 25MPa of CO₂ pressure,50℃ of the temperature, 1.5h of the extraction time and 20L/h of the CO₂ flow rate. Compared with the traditional methods (Water-extracted method and Ethyl alcohol-extracted method), the Supercritical CO₂ Fluid Technology even more manifests to dissolve the organic matters strongly, selectivity good, moreover may in approach to the room temperature to complete the entire isolation and the process is short, which can farthest maintain the component active. Simultaneously the CO₂ gas is very cheap and easy to get and it is nontoxic inert gas to isolate.The thesis also refers to the inhibition of the extracted dihydromyricetin from the Ampelopsis grossedentata on Bacillus subtilis, Escherichia coli, Salmonella typhi, Staphylococcus aureus, Saccharomyces cerevisiae, Enteroacter aerogenes, Aspergillusflavus, Penicillium SP and Aspergilluras Niger, especially to the Bacillus subtilis and Staphylococcus aureus. The minimal inhibitory concentration is 0.0625%.